Effects of Exogenous Substances on Growth of Polyporus umbellatus Mycelium and Its Polysaccharide Content
10.13422/j.cnki.syfjx.20210911
- VernacularTitle:外源性物质对猪苓菌丝体生长及多糖含量的影响
- Author:
Yuan ZHENG
1
;
Yang-hua LI
1
;
Peng-jie HAN
2
;
Yu-yang ZHAO
2
;
Jun-hui ZHOU
2
;
Tie-gui NAN
2
;
Quan YANG
1
;
Min ZHANG
3
;
Yuan YUAN
1
Author Information
1. School of Traditional Chinese Medicine，Guangdong Pharmaceutical University，Guangzhou 510006，China
2. State Key Laboratory of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Hubei Mengyang Pharmaceutical Co. Ltd.，Jingmen 448124，China
- Publication Type:Research Article
- Keywords:
exogenous substances;
Polyporus umbellatus mycelium;
growth rate;
polysaccharide content
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(14):129-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of diverse exogenous substances at different concentrations on the growth of Polyporus umbellatus mycelium and polysaccharide content and screen out the optimal growth condition for P. umbellatus mycelium， so as to provide a reference for its large-scale artificial cultivation. Method:P. umbellatus mycelium was cultured in media containing different exogenous substances using the method for fungal culturing in plate. The growth rate of the mycelium was judged by the colony diameter and the polysaccharide content was determined by the phenol-sulfuric acid method. Result:The high-dose cyclic adenosine monophosphate， 6-benzyl aminopurine （6-BA）， gibberellic acid （GA）， 2，4-dichlorophenoxyacetic acid （2，4-D）， vitamin （V） B1， VB3， VB6， VB9， and VB12 all promoted the growth of P. umbellatus mycelium and elevated polysaccharides content. By contrast， indole acetic acid （IAA）， VC， and VB2 inhibited its growth， with the most obvious inhibition detected in the high-dose VC group. IAA and VB2 both reduced the polysaccharide content， whereas the high-dose VC significantly increased the polysaccharide content. Cyclic adenosine monophosphate， 6-BA， GA， 2，4-D， VB1， VB3， VB6， VB9， and VB12 at the concentrations of 2 mmol·L-1， 6 mg·L-1， 15 mg·L-1， 2 mg·L-1， 4 mg·L-1， 2 mg·L-1， 4 mg·L-1， 6 mg·L-1， and 10 mg·L-1， respectively， contributed to the growth of P. umbellatus mycelium and polysaccharide accumulation. Conclusion:The growth of P. umbellatus mycelium and polysaccharide accumulation can be regulated by adding exogenous substances to the culture medium.
