Mechanism of Astragali Radix Vesicle-like Nanoparticles for Reducing Blood Glucose in db/db Diabetic Mice by Regulating Gut Microbiota
10.13422/j.cnki.syfjx.20211556
- VernacularTitle:黄芪囊泡样纳米颗粒通过调节肠道菌群降低db/db糖尿病小鼠血糖的作用机制分析
- Author:
Wen-jing GAO
1
;
Min HOU
1
;
Xiao-xiao CHEN
1
;
Pan WANG
1
;
Jun-guo REN
1
;
Jian-xun LIU
1
Author Information
1. Beijing Key Laboratory of Pharmacology of Traditional Chinese Medicine， Institute of Basic Medical Sciences，Xiyuan Hospital，China Academy of Chinese Medical Sciences， Beijing 100091，China
- Publication Type:Research Article
- Keywords:
Astragali Radix;
vesicle-like nanoparticles;
ultracentrifugation;
size exclusion chromatography;
fasting blood glucose;
intestinal flora;
particle size distribution
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(14):111-118
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the extraction method and characteristics of vesicle-like nanoparticles （VLNs） in Astragali Radix decoction， and to explore the mechanism of the VLNs in reducing blood glucose by regulating the gut microbiota of db/db diabetic mice. Method:Ultracentrifugation and size exclusion chromatography were used to enrich VLNs from Astragali Radix decoction， and the morphology， particle size and concentration of the VLNs were analyzed by transmission electron microscope and nanoparticle tracking analyzer. The db/db diabetic mice were randomly divided into model group， Astragali Radix VLNs high-， medium- and low-dose （21.1， 10.6， 5.3 g·kg-1） groups and metformin group （0.25 g·kg-1） according to their blood glucose levels. There were 7 mice in each group， and another 7 C57BL/6 mice were set as the normal group. The mice were given intragastrically for 3 weeks （once a day）， and the changes of fasting blood glucose were observed every week. Hematoxylin-eosin （HE） staining was used to observe the pathological morphology of liver and pancreas of diabetic mice. The feces of mice were collected for 16S rRNA diversity detection of intestinal microbes. Result:The size of the nanoparticles obtained by the two methods was about 200 nm. Astragali Radix VLNs extracted by ultracentrifugation had a typical saucer-like shape with the concentration of 3.0×1011 particles·mL-1. The morphology of Astragali Radix VLNs obtained by size exclusion chromatography was relatively poor with the concentration of 2.2×1011 particles·mL-1. After 3 weeks of administration， compared with the model group， Astragali Radix VLNs high-， medium- and low-dose groups could significantly reduce the fasting blood glucose of diabetic mice （P<0.05， P<0.01）. The VLNs could improve the gut microbiota dysbiosis， significantly decrease the ratio of Firmicutes/Bacteroidota， and increase the relative abundance of beneficial bacteria and reduce the relative abundance of harmful bacteria. Conclusion:Astragali Radix VLNs may reduce the blood glucose of db/db diabetic mice by adjusting the ratio of Firmicutes/Bacteroidota in the intestinal flora.
