Regulatory Mechanism of Zedoary Turmeric Oil on VEGFA， STAT3 and mTOR in Ovarian Cancer
10.13422/j.cnki.syfjx.20211491
- VernacularTitle:莪术油对卵巢癌VEGFA，STAT3，mTOR的调控机制
- Author:
Zhi-yong CAO
1
;
Jing-qin CHEN
1
;
Ting LYU
2
;
Chao-jia SHI
1
;
Qiu-yu FENG
1
;
Gang FANG
3
Author Information
1. Key Laboratory of Applied Basic Research of Guangxi Zhuang Medicine，Guangxi University of Chinese Medicine， Nanning 530001，China
2. Medical School， Hubei Minzu University， Enshi 445000， China
3. Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine， Nanning 530001，China
- Publication Type:Research Article
- Keywords:
zedoary turmeric oil;
ovarian cancer （OC）;
curcumol;
curgerenone;
vascular endothelial growth factor A （VEGFA）;
signal transducer and activator of transcription 3 （STAT3）;
mechanistic target of rapamycin （mTOR）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(14):70-80
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects and mechanism of zedoary turmeric oil and its active components on the vascular endothelial growth factor A （VEGFA）， signal transducer and activator of transcription 3 （STAT3）， and mechanistic target of rapamycin （mTOR） in the ovarian cancer （OC）. Method:Network pharmacology technology was employed to analyze the mechanism of Curcumae Rhizoma on OC. Bioinformatics was used to analyze the expression of VEGFA， STAT3， and mTOR in OC and the effect on the prognosis of OC to explore the feasibility of zedoary turmeric oil in regulating VEGFA， STAT3， and mTOR in OC.The xenograft tumor model of nude mice was established， and the effects of zedoary turmeric oil and its active components on VEGFA， STAT3， and mTOR in OC were observed by hematoxylin-eosin （HE） staining， real-time fluorescence-based quantitative polymerase chain reaction （Real-time PCR）， Western blot， and immunohistochemistry （IHC）. Result:Bioinformatics analysis and literature research showed that VEGFA， STAT3， and mTOR played a special regulatory role in the occurrence and development of OC， and were potential key targets for the proliferation of OC. Network pharmacology analysis revealed that Curcumae Rhizoma could regulate multiple disease targets of OC， and mediate VEGFA， STAT3， and mTOR in OC through these multiple targets. As demonstrated by HE staining， the tumor cells in the model group were densely arranged， with no erosion on the edge and no vesicles inside. Compared with the model group， the cell density in other treatment groups was reduced， and strip-shaped erosion on the edge and small empty vesicles were observed in the tumor tissue， especially in the zedoary turmeric oil group. According to the results of Real-time PCR and IHC， zedoary turmeric oil and its active components could inhibit the mRNA and protein expression of VEGFA， STAT3， and mTOR in the OC tissue （P<0.05）. Conclusion:Zedoary turmeric oil and its active components could reduce the expression of VEGFA， STAT3， and mTOR in tumor tissue of nude mice， and inhibited the proliferation of OC through VEGFA， STAT3， and mTOR.
