Analysis on YABBY Transcription Factor Family of Original Plant Cannabis sativa of Traditional Chinese Medicine Cannabis Fructus
10.13422/j.cnki.syfjx.20202115
- VernacularTitle:中药火麻仁基原植物大麻全基因组YABBY转录因子分析
- Author:
Jia-ying SUN
1
;
Zhen WANG
1
;
Yao-lei MI
2
;
Xiang-xiao MENG
2
;
Hui-hua WAN
2
;
Yun YAN
3
;
Wei SUN
2
;
Wei MA
1
Author Information
1. College of Pharmaceutical Sciences，Heilongjiang University of Chinese Medicine，Harbin 150040，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Guizhou Bailing Group Pharmaceutical Co. Ltd.，Anshun 561000，China
- Publication Type:Research Article
- Keywords:
bioinformatics;
Cannabis sativa;
YABBY gene;
conservative analysis;
evolutionary analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(5):124-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the sequence characteristics，chromosomal location，gene structure，conserved motifs，phylogenetic evolution and differential gene expressions of the Cannabis sativa YABBY transcription factor family，in order to provide a molecular basis for in-depth study of YABBY gene function and theoretical support for the selection and breeding of superior hemp varieties. Method:The bio-informatics method was used to identify and analyze the CsYABBY gene family of the original hemp seed plant. PlantTFDB，ExPASy，MEME，CELLO，PLANTCARE and other online websites and TBtools，MEGA，DNAMAN and other software were used for prediction，visualization and analysis. Result:C. sativa contains 6 YABBY gene members distributed on 5 chromosomes，in which 5 members are localized in the nucleus and 1 in extracellular， they consist of 185-235 amino acids， and the isoelectric point is between 5.05 and 9.34， the molecular weight is between 20 582.45-26 282.7 Da. All of CsYABBY proteins contain two conserved domains， namely Zinc finger domain and YABBY domain. CsYABBY genes have multiple cis-acting elements，and their expressions differ in different tissues and cultivars. Conclusion:The expressions of CsYABBY may be affected by hormones and externally environmental factors. CsYABBY gene expressions are tissue-specific. In addition，YABBY transcription factor family may play an important role in regulating the development of C. sativa female flowers，and subfamilies YAB1 and YAB5 may be involved in the synthesis of cannabinoids.
