Effect of Dunhuang Pingweiwan and Its Decomposed Recipes on Tumor Inhibition and PI3K/Akt/mTOR Pathway in SCG-7901 Gastric Cancer Mice
10.13422/j.cnki.syfjx.20202229
- VernacularTitle:敦煌平胃丸及其拆方对SCG-7901胃癌荷瘤小鼠的抑瘤作用及对PI3K/Akt/mTOR信号通路的影响
- Author:
Ya-li SHE
1
;
Xiao-wen ZHAO
1
;
Jun-jie LI
1
;
Juan-juan DONG
1
;
Yong-qi LIU
1
;
Xi-ping LIU
1
;
Chang-tian LI
1
;
Ya-ling LI
1
Author Information
1. Key Laboratory of Dunhuang Medicine， Ministry of Education， Provincial Key Laboratory for Molecular Medicine of Major Diseases and Traditional Chinese Medicine Prevention and Treatment Research， Gansu University of Chinese Medicine， Lanzhou 730000， China
- Publication Type:Research Article
- Keywords:
Dunhuang Pingweiwan;
gastric cancer;
phosphatidylinositol 3-kinase （PI3K）;
protein kinase B （Akt）;
mammalian target of rapamycin （mTOR）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(5):70-76
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the antitumor effect and the mechanism of Dunhuang Pingweiwan and its decomposed recipes based on phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt）/mammalian target of rapamycin （mTOR） signaling pathway in SCG-7901 gastric cancer-mice. Method:The subcutaneous tumor bearing model of SCG-7901 gastric cancer in mice was established， and the the mice were randomized into model group， Dunhuang Pingweiwan group （14.04 g·kg-1·d-1）， Huoxue Jiedu group （6.50 g·kg-1·d-1）， Wenzhong Sanhan group （3.64 g·kg-1·d-1） and cisplatin group （2 mg·kg-1·d-1）， with 8 mice in each group. From the 8th day of inoculation， the mice were administered for 10 consecutive days. The mice were weighed and the general conditions were observed every other day. On the next day of the last administration， the mice were sacrificed， and the tumor was removed and weighed to calculate the anti-tumor rate. The histopathological changes were observed by hematoxylin-eosin （HE） staining， and the mRNA and protein expressions of PI3K， Akt， and mTOR in tumor tissues were detected by real-time polymerase chain reaction（Real-time PCR） and immunohistochemistry （IHC）， respectively. Result:From the 10th day of inoculation， the mice in cisplatin group were generally in poor condition and their body mass decreased. The mice in model group， Dunhuang Pingweiwan group， Huoxue Jiedu group and Wenzhong Sanhan group were generally fair， and their body mass increased without significant difference among groups. The tumor inhibition rates of Dunhuang Pingweiwan， Huoxue Jiedu， Wenzhong Sanhan and cisplatin groups were 30.74%， 24.80%， 4.19% and 63.84%， respectively. Except for Wenzhong Sanhan group， tumor weight of the other treatment groups was significantly lower than that of the model group （P<0.01）， and there was no significant difference between the Dunhuang Pingweiwan and Huoxue Jiedu group. Dunhuang Pingweiwan and Huoxue Jiedu group could significantly reduce tumor cell density and cause tumor cell necrosis. Compared with the model group， the expressions of PI3K， Akt， and mTOR mRNA and protein in the Dunhuang Pingweiwan， Huoxue Jiedu and cisplatin groups significantly decreased （P<0.05， P<0.01）， and there was no significant difference between the Dunhuang Pingweiwan group and Huoxue Jiedu group. Conclusion:Dunhuang Pingweiwan and its decomposed recipes （Huoxue Jiedu） have a certain anti-tumor effect on the SCG-7901 gastric cancer-mice， and the mechanism may be related to the down-regulation of key molecules in the PI3K/Akt/mTOR signaling pathway.
