Effect of Shaoyaotang on Expressions of CD14, FADD and Caspase-8 in Colonic Tissues of Rats with Large Intestinal Damp-heat Syndrome of Ulcerative Colitis
10.13422/j.cnki.syfjx.20202401
- VernacularTitle:芍药汤对大肠湿热型溃疡性结肠炎大鼠结肠组织中CD14，FADD，Caspase-8表达的影响
- Author:
Si-qi CAO
1
;
Feng-yi WANG
1
;
Sheng-nan TANG
1
;
Dang-sheng ZHAO
1
;
Yang-yang LI
1
;
Zhi-jie LIU
1
;
Rui-ting CHAI
1
Author Information
1. Gansu University of Chinese Medicine， Lanzhou 730000， China
- Publication Type:Research Article
- Keywords:
Shaoyaotang;
ulcerative colitis;
leukocyte differentiation antigen 14 （CD14）;
Fas-related death domain protein （FADD）;
cysteinyl aspartate specific protease-8 （Caspase-8）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(5):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Shaoyaotang on the contents of cell adhesion molecule-1 （ICAM-1） and transforming growth factor-β1 （TGF-β1） in serum of large intestine damp-heat syndrome of ulcerative colitis （UC） in rats， and the gene and protein expressions of leukocyte differentiation antigen14 （CD14）， Fas-related death domain protein （FADD） and cysteinyl aspartate specific protease-8 （Caspase-8） in the focal colon tissue. Method:A total of 80 SPF Wistar rats were randomly divided into the blank group （n=10） and modeling group （n=70）. The large intestine damp-heat syndrome of UC rats was replicated by the combination of disease and syndrome， which was high-fat， high-sugar and spicy diets combined with 2， 4-dinitrobenzene sulfonic acid （DNBS） and ethanol. After successful modeling， the modeled groups were divided into model group， sulfasalazine （SASP）control group， and low， medium and high-dose Shaoyaotang groups by the method of random number table， with14 rats in each group. Low， medium and high doses of Sulfasalazine 0.2 g·kg-1·d-1 and Shaoyaotang （6， 12， 24 g·kg-1·d-1）were given by gavage. The blank group and the model group were given equal volume of normal saline for 21 days. The contents of serum ICAM-1 and TGF-β1 were detected by enzyme-linked immunosorbent assay （ELISA）， the expressions of CD14， FADD and Caspase-8 mRNA in colon tissues were detected by Real-time quantitative polymerase chain reaction （Real-time PCR）， and the expressions of CD14， FADD and Caspase-8 protein in colon tissues were detected by Western blot. Result:Compared with the blank group， the serum ICAM-1 level in the model group were significantly increased， whereas the content of TGF-β1 were significantly decreased （P<0.05）. The relative expression levels of CD14， FADD， Caspase-8 mRNA and protein were significantly increased （P<0.05）. Compared with the model group， the content of ICAM-1 in the serum of the rats in the medium， high-dose Shaoyaotang groups and the SASP group were significantly decreased， while the content of TGF-β1 in the serum of the rats in the low， medium， high-dose Shaoyaotang groups and the SASP group were significantly increased （P<0.05）. The expression levels of CD14， FADD， Caspase-8 mRNA and protein in each intervention group were significantly decreased （P<0.05）， especially in the high-dose Shaoyaotang group and the SASP group. Conclusion:Shaoyaotang has a certain intervention effect on UC rats with large intestine damp-heat syndrome， and its mechanism may be related to the inhibition of CD14， FADD and Caspase-8 genes and proteins expression.
