Effect of Compatibility of Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex Couplet Medicines on Glucolipid Metabolism in Type 2 Diabetic Rats Before and After Salt-processing
10.13422/j.cnki.syfjx.20202048
- VernacularTitle:知母-黄柏药对盐炙前后配伍对2型糖尿病大鼠糖脂代谢的影响
- Author:
Shun-ming FAN
1
;
Chun-ling ZHANG
1
;
Jia-qi WANG
1
;
Xing LI
1
;
Ting LUO
1
;
Ling-ying YU
1
Author Information
1. Key Laboratory of Standardization of Chinese Herbal Medicine，Ministry of Education， College of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
- Publication Type:Research Article
- Keywords:
couplet medicines;
Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex;
processed with salt;
type 2 diabetes mellitus （T2DM）;
glucolipid metabolism;
kidney function;
adipocytokines
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(3):22-29
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of compatibility of Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex couplet medicines on glucolipid metabolism in type 2 diabetic rats before and after salt-processing. Method:The type 2 diabetic rat model was induced by high-fat and high-glucose diet combined with low dose streptozotocin （STZ）， the model rats were randomly divided into six groups， including the model group， metformin group （200 mg·kg-1）， and different compatibility groups of raw and salt-processed of Anemarrhenae Rhizoma and Phellodendri Chinensis Cortex （6.48 g·kg-1）. In addition， The same week old rats fed with normal diet were set as the blank group. After 30 d of continuous intragastric administration， changes of fasting blood glucose （FBG）， fasting serum insulin （FINS）， glycosylated serum protein （GSP）， hepatic glycogen， blood lipid ［total cholesterol （TC）， triglyceride （TG）， low density lipoprotein cholesterol （LDL-C）， high density lipoprotein cholesterol （HDL-C）］， nonesterified fatty acid （NEFA）， adipocytokines ［adiponectin （ADP） and leptin）］， kidney function ［blood urea nitrogen （BUN） and creatinine （CRE）］ and other indicators of rats from different groups were detected， and the insulin sensitivity index （ISI） and insulin resistance index （HOMA-IR） were calculated， hematoxylin-eosin （HE） staining was used to observe the morphological changes of pancreas， liver and kidney of rats from different groups. Result:Compared with the model group， compatibility of Anemarrhenae Rhizoma-Phellodendri Chinensis Cortex couplet medicines before and after salt-processing all could decrease the levels of FBG， GSP， TC， TG， LDL-C， NEFA， leptin， BUN， CRE and HOMA-IR， and increase the contents of FINS， HDL-C， ADP， hepatic glycogen and ISI， among which the compatibility of salt-processed Anemarrhenae Rhizoma and salt-processed Phellodendri Chinensis Cortex had the most significant effect on regulating glucolipid metabolism in type 2 diabetic rats. The compatibility of all couplet medicines could improve the histopathological changes of pancreas， liver and kidney in type 2 diabetic rats， among which the compatibility of salt-processed Anemarrhenae Rhizoma and salt-processed Phellodendri Chinensis Cortex had the most prominent effect on repairing pathological damage. Conclusion:The compatibility of Anemarrhenae Rhizoma and Phellodendri Chinensis Cortex before and after salt-processing can improve glucolipid metabolism in type 2 diabetic rats， while the comprehensive effect of salt-processed Anemarrhenae Rhizoma and salt-processed Phellodendri Chinensis Cortex on lowering glucose and regulating lipid is the best.
