Effect of Fangji Huangqitang on Function of Human Umbilical Vein Endothelial Cells
10.13422/j.cnki.syfjx.20210239
- VernacularTitle:防己黄芪汤对脐静脉内皮细胞功能的影响
- Author:
Lian-hua HE
1
;
Qing-xia QIN
1
;
Qing WU
2
;
Hui-jie LUAN
1
;
Juan HE
1
;
Wen-cheng HE
1
;
Qing-wen WANG
1
Author Information
1. Shenzhen Hospital，Peking University， Shenzhen 518036，China
2. Hejiang Hospital of Traditional Chinese Medicine，Sichuan 646200，China
- Publication Type:Research Article
- Keywords:
Fangji Huangqitang;
human umbilical vein endothelial cells;
migration;
invasion;
adhesion;
tube formation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(2):8-14
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of Fangji Huangqitang（FJHQT） on migration， adhesion，invasion and tube formation of human umbilical vein endothelial cells （HUVECs） induced by vascular endothelial growth factor （VEGF）. Method:HUVECs were induced by VEGF （20 μg·L-1） in vitro. The effects of FJHQT （0.25，0.5，1 g·L-1） on HUVECs were detected by methyl thiazolyl tetrazolium（MTT）， scratch repair， transwell migration， adhesion， invasion and tube formation. Protein in HUVECs was extracted and protein expression levels of phosphorylated Janus kinase 1 （p-JAK1） were detected by Western blot. Result:Compared with control group， VEGF （20 μg·L-1） can increase the proliferation， scratch repair， transwell migration， adhesion， invasion and tube formation of HUVECs cells （P<0.01）， compared with VEGF group， FJHQT （0.25，0.5，1 g·L-1） ，there is no significant effect on the proliferation of HUVECs induced by VEGF for 24 hours， but it can significantly reduce the scratch repair， migration， adhesion， invasion and tube formation of HUVECs induced by VEGF within 24 hours （P<0.05）. Compared with blank group， VEGF could induce abnormal elevation of p-JAK1 in HUVECs （P<0.01）， while FJHQT （0.25，0.5，1 g·L-1） could significantly reduce the expression levels of p-JAK1 （P<0.05，P<0.01）. Conclusion:FJHQT can inhibit the migration， adhesion and invasion of HUVECs， the mechanism may be related to JAK1.
