Curcumin reduces cold storage-induced damage in human cardiac myoblasts.
- Author:
Hadil ABUARQOUB
1
;
Colin J GREEN
;
Roberta FORESTI
;
Roberto MOTTERLINI
Author Information
1. Vascular Biology Unit, Department of Surgical Research, Northwick Park Institute for Medical Research, Harrow, Middlesex, United Kingdom. r.motterlini@imperial.ac.uk.
- Publication Type:Original Article
- Keywords:
cryopreservation curcumin;
cytoprotection;
heme oxygenase-1;
organ preservation;
organ transplantation;
oxidative stress
- MeSH:
Cell Death/drug effects;
Cell Survival/drug effects;
Cells, Cultured;
Cold Temperature;
*Cryopreservation;
Cryoprotective Agents/pharmacology;
Curcumin/*pharmacology;
Gene Expression Regulation, Enzymologic/drug effects;
Heme Oxygenase-1/genetics/metabolism;
Hemin/pharmacology;
Humans;
Hydrogen Peroxide/pharmacology;
Myoblasts, Cardiac/*drug effects/*pathology;
RNA, Messenger/genetics/metabolism
- From:Experimental & Molecular Medicine
2007;39(2):139-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
Curcumin is a polyphenolic compound possessing interesting anti-inflammatory and antioxidant properties and has the ability to induce the defensive protein heme oxygenase-1 (HO-1). The objective of this study was to investigate whether curcumin protects against cold storage-mediated damage of human adult atrial myoblast cells (Girardi cells) and to assess the potential involvement of HO-1 in this process. Girardi cells were exposed to either normothermic or hypothermic conditions in Celsior preservation solution in the presence or absence of curcumin. HO-1 protein expression and heme oxygenase activity as well as cellular damage were assessed after cold storage or cold storage followed by re-warming. In additional experiments, an inhibitor of heme oxygenase activity (tin protoporphyrin IX, micrometer) or siRNA for HO-1 were used to investigate the participation of HO-1 as a mediator of curcumin- induced effects. Treatment with curcumin produced a marked induction of cardiac HO-1 in normothermic condition but cells were less responsive to the polyphenolic compound at low temperature. Cold storage-induced damage was markedly reduced in the presence of curcumin and HO-1 contributed to some extent to this effect. Thus, curcumin added to Celsior preservation solution effectively prevents the damage caused by cold- storage; this effect involves the protective enzyme HO-1 but also other not yet identified mechanisms.
