Effect of Ginseng Radix et Rhizoma-Notoginseng Radix et Rhizoma-Chuanxiong Rhizoma Extract on HUVECs Senescence Mediated by Endothelial Microparticles
10.13422/j.cnki.syfjx.20210506
- VernacularTitle:人参-三七-川芎提取物对内皮微粒介导HUVECs衰老的干预作用
- Author:
Bo-wen YU
1
;
Yan-hong HU
1
;
Jing YANG
1
;
Cheng-kui XIU
1
;
Xue WANG
1
;
Yan LEI
1
Author Information
1. Beijing Key Laboratory of Research of Chinese Medicine on Prevention and Treatment for Major Diseases， Medical Experiment Center， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Research Article
- Keywords:
endothelial microparticles;
senescence;
Ginseng Radix et Rhizoma-Notoginseng Radix et Rhizoma-Chuanxiong Rhizoma extract;
reactive oxygen species （ROS）;
human umbilical vein endothelial cells （HUVECs）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(8):50-56
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Ginseng Radix et Rhizoma-Notoginseng Radix et Rhizoma-Chuanxiong Rhizoma extract on endothelial microparticles （EMPs）-induced vascular endothelial cell senescence， and explore the possible mechanism. Method:Human umbilical vein endothelial cells （HUVECs） were used as the research objects， and the aged model was established with 10-12 passages of replicative senescence cells. The experimental cells were divided into young group （2-4 passage cells）， aged group （10-12 passage cells）， only EMPs intervention group （extract EMPs produced by aged cells to intervene young cells） and low dose， middle dose and high dose drug intervention groups （200， 300， 400 mg·L-1）. Senescence related β-galactosidase （SA-β-gal） staining and cell cycle propidium iodide （PI） staining were used to determine cell senescence. Cell counting kit-8 （CCK-8） assay was used to screen the drug concentration. EMPs were extracted by two-step centrifugation， EMPs labeled with phycoerythrin （PE） anti-human CD31 antibody or fluorescein isothiocyanate （FITC） annexin V were detected by flow cytometry， intracellular reactive oxygen species （ROS） were detected by 2'，7'- dichlorofluorescein diacetate （DCFDA） staining. Result:After treatment with the drug， SA-β-gal activity of the aged cells significantly decreased （P<0.01）， the S phase arrest was restored （P<0.01）， and the number of CD31+ EMPs and annexin V+ EMPs secreted by aged cells decreased （P<0.05）. Compared with the young group， only EMPs intervention group could induce increased SA-β-gal activity and S phase arrest in young cells （P<0.05，P<0.01）. However， after intervention of EMPs and the drug， EMPs-mediated increase of SA-β-gal activity was significantly inhibited and S phase arrest was restored （P<0.05）. The increase of intracellular ROS induced by EMPs was also significantly inhibited by the drug （P<0.05，P<0.01）. Conclusion:Ginseng Radix et Rhizoma-Notoginseng Radix et Rhizoma-Chuanxiong Rhizoma extract can delay the senescence of vascular endothelial cells by influencing EMPs， and the mechanism may be related to the inhibition of increased intracellular ROS induced by EMPs.
