Effect of Huangqintang on Oxidative Stress and Ferroptosis-related Indexes GSH-Px4，P53，SLC7A11 in Ulcerative Colitis Mice
10.13422/j.cnki.syfjx.20210505
- VernacularTitle:黄芩汤对溃疡性结肠炎小鼠氧化应激及铁死亡相关指标GSH-Px4，P53，SLC7A11的影响
- Author:
Na WU
1
;
Zhi-ping WAN
2
;
Juan ZENG
1
;
Hui-ying LIU
3
;
Xiao-ping HE
4
;
Xian-hui DONG
4
Author Information
1. Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine（TCM），Nanchang 330006，China
2. Nanchang Hongdu Hospital of TCM，Nanchang 330006，China
3. Jiangzhong Pharmaceutical Co. Ltd.，Nanchang 330004，China
4. Hebei University of Chinese Medicine，Shijiazhuang 050091，China
- Publication Type:Research Article
- Keywords:
Huangqintang;
ulcerative colitis;
ferroptosis;
oxidative stress
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(8):17-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the possible mechanism of Huangqintang in treating ulcerative colitis （UC）. Method:The animal model of UC was induced by dextran sodium sulfate （DSS）．The experimental animals were divided into control group， model group，Huangqintang low dose （4.55 g·kg-1）， medium dose （9.1 g·kg-1）， and high dose（18.2 g·kg-1） groups. Intragastric administration was also given in the modeling process for 7 consecutive days. At the end of the 8th day， colon tissues were collected to measure colon length and mass， and calculate the colon mass index. Pathological changes were observed by hematoxylin-eosin （HE） staining. Serum iron content， superoxide dismutase （SOD）， glutathione （GSH）， catalase （CAT） and myeloperoxidase （MPO） were determined by biochemical assay. Western blot was used to detect the protein expression of glutathione peroxidase 4 （GSH-Px4）， long-chain acyl-CoA synthetase 4 （ACSL4） and ferritin heavy chain 1（FTH1）. The mRNA expression levels of tumor trotein 53 （P53） and solute carrier family 7 member 11 （SLC7A11） in colon tissues were detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR）. Result:The experimental studies showed that compared with normal group， serum MPO and iron content， ACSL4 protein level and relative P53 mRNA expression in the model group significantly increased （P<0.05）， while serum SOD， CAT， GSH content， GSH-Px4， FTH1 relative protein expression level and relative SLC7A11 mRNA expression in the model group significantly decreased （P<0.01）. Compared with model group， serum MPO and iron content， ACSL4 protein level and relative P53 mRNA expression significantly decreased （P<0.05）， while serum SOD， CAT， GSH content， GSH-Px4， FTH1 relative protein expression level and relative SLC7A11 mRNA expression significantly increased （P<0.05） after the intervention of Huangqintang， and the effect was most significant in the high-dose group （P<0.05）. The results of general condition， colon length， colon mass index and HE staining showed that Huangqintang could relieve clinical symptoms and histopathological changes in UC mice. Conclusion:These results indicated that Huangqintang had therapeutic effect on ulcerative colitis mice， and its mechanism might be related to inhibiting the oxidative stress and ferroptosis.
