Analysis of Chemical Constituents in Qizhi Jiangtang Capsules Based on UPLC-QE-Orbitrap-MS
10.13422/j.cnki.syfjx.20210851
- VernacularTitle:基于UPLC-QE-Orbitrap-MS技术的芪蛭降糖胶囊化学成分分析
- Author:
Jing-chao SHI
1
;
Shu-rong ZHANG
1
;
Zhi CHAI
1
;
Xiang-jun CHEN
1
;
Wei-zheng ZHANG
1
Author Information
1. School of Chinese Materia Medica and Food Engineering， Shanxi University of Chinese Medicine，Jinzhong 030619，China
- Publication Type:Research Article
- Keywords:
Qizhi Jiangtang capsules;
ultra performance liquid chromatography-quadrupole-electrostatic field orbital trap high resolution mass spectrometry （UPLC-QE-Orbitrap-MS）;
chemical constituents;
fragmentation pattern;
flavonoids;
iridoid glycosides;
phenylpropanoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(16):116-123
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To systematically analyze the chemical constituents of Qizhi Jiangtang capsules by ultra performance liquid chromatography-quadrupole-electrostatic field orbital trap high resolution mass spectrometry （UPLC-QE-Orbitrap-MS）. Method:Analysis was conducted on a ACQUITY UPLC HSS T3 column （2.1 mm×100 mm， 1.8 μm） with acetonitrile （A）-water （B） as the mobile phase for gradient elution （0-13 min， 1%-25%A； 13-21 min， 25%-35%A； 21-28 min， 35%-85%A； 28-30 min， 85%-100%A； 30-32 min， 100%-1%A）. The flow rate was 0.2 mL·min-1， the column temperature was 30 ℃， and the volume of sample injection was 3 μL. Electrospray ionization （ESI） was used to collect data in the negative and positive ion modes with the scanning range of m/z 100-1 500. Meanwhile， a variety of MS analytic methods were used， including comparing with the information of control substances， self-built compounds database and literature references， diagnostic ion filtering， Compound Discoverer 3.0 software， for identification of the chemical components. Result:Based on the above strategy， a total of 52 compounds were identified in Qizhi Jiangtang capsules， and the sources of these compounds were identified. Amino acids were mainly derived from Hirudo， phenylpropanoids were derived from Astragali Radix and Rehmanniae Radix， iridoid glycosides were derived from Rehmanniae Radix， coumarins and triterpenes were derived from Astragali Radix， flavonoids were from Astragali Radix and Polygonati Rhizoma. Conclusion:The established UPLC-QE-Orbitrap-MS analytical method can comprehensively and rapidly analyze and identify of the chemical constituents in Qizhi Jiangtang capsules. Many of the ingredients have been proved by modern pharmacological studies to have the effect of improving related symptoms of diabetes and its complications， reflecting the characteristics of synergistic action of multiple components in Qizhi Jiangtang capsules. This study can provide reference for the further research on the pharmacodynamic material basis and the quality control of Qizhi Jiangtang capsules.
