Protective Effect of Angelicae Sinensis Radix-Chuanxiong Rhizoma Medicated Serum Against H2O2-induced Oxidative Damage of PC12 Cells Based on Nrf2/ARE Signaling Pathway
10.13422/j.cnki.syfjx.20211506
- VernacularTitle:基于Nrf2/ARE信号通路探讨当归-川芎含药血清对H2O2致PC12细胞氧化损伤的保护作用
- Author:
Man-xue YIN
1
;
Yu-jie LIN
2
;
Wen-zhi HUANG
3
;
Si-jun LIU
1
;
Xiao-lan ZHOU
1
;
Qing-guang WU
1
Author Information
1. School of Pharmaceutical Sciences，Guangzhou University of Chinese Medicine，Guangzhou 510006，China
2. The Second Clinical Medical College，Guangzhou University of Chinese Medicine，Guangzhou 510006，China
3. Guangdong Provincial Hospital of Chinese Medicine，Guangzhou 510006，China
- Publication Type:Research Article
- Keywords:
Angelicae Sinensis Radix;
Chuanxiong Rhizoma;
medicated serum;
oxidative damage;
nuclear factor E2-related factor 2 （Nrf2）/antioxidant response element （ARE） signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(16):67-74
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect and molecular mechanism of Angelicae Sinensis Radix-Chuanxiong Rhizoma medicated serum （ASRCRS） against oxidative damage of PC12 cells induced by H2O2. Method:Oxidative damage of PC12 cells was induced by H2O2 in vitro， and intervention was performed in the low-， medium-， and high-dose ASRCRS groups with a final volume fraction of 15%. The cell viability was determined by methyl thiazolyl tetrazolium （MTT） assay. Cell morphology was observed by an inverted fluorescence microscope. The content of lactate dehydrogenase （LDH） and malondialdehyde （MDA）， the activity of superoxide dismutase （SOD）， and the distribution of reactive oxygen species （ROS） in the cell supernatant were detected by the kits. Cell apoptosis was detected by Annexin V-FITC/PI double staining. The protein expression levels of nuclear factor E2-related factor 2 （Nrf2）， Kelch-like epichlorohydrin associated protein-1 （Keap1）， heme oxygenase-1 （HO-1）， and SOD1 were detected by Western blot. Result:Oxidative damage was induced by 300 μmol·L-1 H2O2 for 24 hours. Compared with the normal group， the model group showed abnormal cell morphology， reduced cell viability （P<0.01）， increased LDH and MDA （P<0.01）， blunted SOD activity， elevated intracellular distribution of ROS， down-regulated protein expression of Nrf2， HO-1， and SOD1 （P<0.05， P<0.05）， and up-regulated protein expression of Keap1 （P<0.01）. Compared with the model group， ASRCRS groups displayed improved cell morphology， increased cell viability， inhibited cell apoptosis， potentiated SOD activity （P<0.01）， suppressed release of LDH （P<0.01） and generation of ROS， decreased content of MDA （P<0.01）， up-regulated protein expression of Nrf2， HO-1 and SOD1 （P<0.05， P<0.01）， and down-regulated protein expression of Keap1 （P<0.01）. Conclusion:ASRCRS could protect PC12 cells from oxidative damage induced by H2O2 by up-regulating the expression of Nrf2 to activate the Nrf2/antioxidant response element （ARE） signaling pathway， enhancing the ability to resist oxidative damage， and inhibiting cell apoptosis.
