Mechanism of Quercetin in Protecting Articular Cartilage from Osteoarthritis Based on p38 MAPK Pathway
10.13422/j.cnki.syfjx.20192084
- VernacularTitle:基于p38 MAPK信号通路分析槲皮素保护骨性关节炎关节软骨的机制
- Author:
Xiang-peng WANG
1
;
Wen-peng XIE
2
;
Yi-fei BI
3
;
Bao-an WANG
4
;
Rong-xiu BI
1
Author Information
1. Shandong University of Traditional Chinese Medicine（TCM），Ji'nan 250355，China
2. Affiliated Hospital of Shandong University of TCM，Ji'nan 250014，China
3. Medical College of Optometry and Ophthalmology of Shandong University of TCM，Ji'nan 250002，China
4. Shandong Institute of Commerce and Technology，Ji'nan 250103，China
- Publication Type:Research Article
- Keywords:
quercetin;
network pharmacology;
p38 mitogen activated protein kinase （MAPK） signaling pathway;
osteoarthritis;
protective mechanism
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(7):169-177
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the protective effect of quercetin （Qu） on articular cartilage of knee osteoarthritis and its mechanism by inhibiting p38 mitogen activated protein kinase （MAPK） signaling pathway. Method:Through the network pharmacology technology，we scientifically predicted and analyzed the target factors and signal pathways of Qu in the protection of articular cartilage in patients with osteoarthritis. We selected a prediction pathway closely related to osteoarthritis and validated it by cell experiment in vitro. The best intervention concentration of the drug was selected by cell counting kit-8 （CCK-8） method. The osteoarthritis and its closely related inflammatory factors interleukin（IL）-1β and tumor necrosis factor（TNF）-α were detected by enzyme linked immunosorbent assay（ELISA）. The expression of related mRNA and protein in p38 signal pathway after Qu intervention were detected by quantitative real time polymerase chain reaction（Real-time PCR） and Western blot. Result:It was predicted that MAPK signal pathway was closely related to osteoarthritis by network pharmacology，and p38 MAPK pathway，which was most closely related to osteoarthritis，was selected for study. The results showed that 100 μmol·L-1 Qu had the most obvious effect in decreasing the expression of related inflammatory factors，inhibited the expression of p38，phosphorylated（p）-p38，matrix metalloproteinase-13（MMP-13），A disintegrin-like and metalloproteinase with thrombospondin type-1 motifs-4（ADAMTS-4） in the pathway，and promoted the expression of CollagenⅡ. Conclusion:Qu could decrease the expression of cartilage inflammatory factors in the prevention and treatment of osteoarthritis，and the effect can be well developed by intervening and inhibiting p38 MAPK pathway related factor expression level. All the results show that Qu can decrease osteoarthritis inflammatory factors and protect articular cartilage in patients with osteoarthritis.
