Resveratrol stimulates glucose transport in C2C12 myotubes by activating AMP-activated protein kinase.
- Author:
Chang Eun PARK
1
;
Min Jung KIM
;
Jong Hwa LEE
;
Byung Il MIN
;
Hyunsu BAE
;
Wonchae CHOE
;
Sung Soo KIM
;
Joohun HA
Author Information
1. Department of East-West Medicine, Graduate School, Kyung Hee University, Seoul 130-701, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
1-Phosphatidylinositol 3-Kinase/metabolism;
AMP-Activated Protein Kinases;
Animals;
Biological Transport/drug effects;
Cell Line;
Enzyme Activation/drug effects;
Glucose/*metabolism;
Insulin/metabolism;
Mice;
Models, Biological;
Multienzyme Complexes/*metabolism;
Muscle Fibers, Skeletal/*drug effects/enzymology/metabolism;
Protein-Serine-Threonine Kinases/*metabolism;
Proto-Oncogene Proteins c-akt/metabolism;
Stilbenes/*pharmacology
- From:Experimental & Molecular Medicine
2007;39(2):222-229
- CountryRepublic of Korea
- Language:English
-
Abstract:
trans-Resveratrol (t-RVT), a naturally occurring polyphenol found in Polygonum cuspidatum, grape, and red wine, has been reported to have anti- inflammatory, cardioprotective, and cancer chemopreventive properties. However antidiabetic effect of t-RVT has not yet been reported. In this study, we show that t-RVT increases glucose uptake in C2C12 myotubes by activating AMP-activated protein kinase (AMPK), uncovering an antidiabetic potential of t-RVT for the first time. AMPK plays a central role in the regulation of glucose and lipid metabolism, and hence it is considered a novel therapeutic target for metabolic syndrome such as type 2 diabetes. t-RVT significantly induced glucose uptake in C2C12 cells, via AMPK activation, but not a phosphatidylinositol-3 kinase (PI-3 kinase) signal pathway. The induced glucose uptake was attenuated by pretreatment with a pharmacological inhibitor for AMPK, indicating that the effect of t-RVT primarily depends on AMPK activation. However, in the presence of insulin, t-RVT also potentiated the effect of insulin on glucose uptake via AMPK activation, which led to further activation of PI-3 kinase/Akt signal pathway.
