Construction of Evaluating Method and Experimental Research for Targeting of Traditional Chinese Medicine Preparations Based on Supramolecular Qi Chromatography Theory
10.13422/j.cnki.syfjx.20211451
- VernacularTitle:基于超分子“气析”理论构建中药制剂靶向性评价方法及实验验证
- Author:
Min-cun WANG
1
;
Chao HU
1
;
Yu-ting HE
1
;
Yan-zi ZHOU
1
;
You-zhi LIU
1
;
Qi-meng FAN
1
;
Ding-fang CHEN
1
;
Hai-ying LI
1
;
Xue PAN
1
;
Yi-qun ZHOU
1
;
Fu-yuan HE
1
Author Information
1. Laboratory of Supramolecular Mechanism and Mathematic-physics Chracterization for Traditional Chinese Medicine，Hunan Provincial Key Laboratory of Druggability and Preparation Modification for ChineseMateria Medica，School of Pharmacy， Hunan University of Chinese Medicine，Changsha 410208，China
- Publication Type:Research Article
- Keywords:
traditional Chinese medicine;
supramolecular Qi chromatography theory;
molecular connectivity index;
liver targeting;
Pien Tze Huang;
Bupleuri Radix;
imprinted template
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(20):113-120
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the targeting evaluation method of traditional Chinese medicine （TCM） preparations based on supramolecular Qi chromatography theory， and to study the liver targeting effect of Bupleuri Radix on Pien Tze Huang. Method:The molecular connectivity index （MCI） was used to analyze the characteristics of imprinted template and liver targeting tendency of TCM mainly attributed to liver meridian and components of Pien Tze Huang， and combined with target dynamics and total statistical moment principle， aimed at the independent action characteristics of multi-component imprinted template of TCM， a method for evaluating the targeting of TCM preparations was established. Hepatoma rats in Pien Tze Huang group， Bupleuri Radix group， Pien Tze Huang+Bupleuri Radix group and blank group were tested and verified. Result:After the average value of MCI of TCM mainly attributed to liver meridian was deducted， the MCI similarity between Pien Tze Huang group and Bupleuri Radix group was 0.376 8， Pien Tze Huang+Bupleuri Radix group and Bupleuri Radix group was 0.988 2， so it was predicted that Bupleuri Radix could enhance the liver targeting of Pien Tze Huang. A system for evaluating the targeting of TCM compounds was established， including relative total uptake efficiency （RUET）， relative total concentration （RCT）， relative imprinted tendency （RITT） and relative imprinted variance （RIVT）. The RUET and RCT of liver were the highest in all tissues （RUET=1.88>1，RCT=2.30>1）， and the corresponding values of other tissues were all <1， indicating that Pien Tze Huang combined with Bupleuri Radix could increase its distribution in liver and enhance liver targeting. Except for plasma， the RITT and RIVT of other tissues fluctuated around 1.0， indicating that targeted modification did not change imprinted tendency of Pien Tze Huang and had no significant effect on the types of components. Conclusion:Under the guidance of supramolecular Qi chromatography theory， a targeting evaluation parameter system can be established to characterize the multi-component imprinted effect of TCM preparations by MCI and total statistical moment parameters， so as to realize the evaluation of targeting of TCM preparations. The addition of Bupleuri Radix can increase the liver targeting of Pien Tze Huang.
