Inhibitory Effect of Duanteng Yimu Decoction on Pannus Formation in Mice with Collagen-induced Arthritis Based on VEGF Pathway
10.13422/j.cnki.syfjx.20211707
- VernacularTitle:基于VEGF通路探讨断藤益母汤抑制胶原诱导型关节炎小鼠血管翳形成的作用
- Author:
Kai QIAN
1
;
Li-ying ZENG
1
;
Xue-xia ZHENG
1
;
Shu-di XU
1
;
Dan-kai LIU
1
;
Jing-yi ZHAN
1
;
Tang-ming YE
1
;
Fa-jie LIAO
1
;
Min-ying LIU
2
;
Chang-song LIN
2
Author Information
1. Guangzhou University of Chinese Medicine，Guangzhou 510405，China
2. The First Affiliated Hospital of Guangzhou University of Chinese Medicine，Guangzhou 510405，China
- Publication Type:Research Article
- Keywords:
Duanteng Yimu decoction;
collagen-induced arthritis;
pannus;
vascular endothelial growth factor （VEGF） signaling pathway;
CD31
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(20):63-71
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the mechanism of Duanteng Yimu decoction （DTYM） in the inhibition of pannus formation in collagen-induced arthritis （CIA） mice. Method:Twenty-four SPF-grade DBA/1 male mice were randomly divided into the following four groups： a blank group （NC group）， a model group （CIA group）， a methotrexate group （MTX group）， and a DTYM group， with six mice in each group. The mice， except for those in the NC group， were modeled. From the second immunization， the medium， MTX （1 mg·kg-1）， and DTYM （15.4 g·kg-1） were administered at an equal volume by gavage for 35 days. Mice were observed for general condition and the arthritis index. The knee and ankle joints were scanned by microcomputed tomography （micro CT）. Hematoxylin-eosin （HE） and safranin O/fast green staining were performed to observe pathological changes. Immunohistochemistry was performed to detect the expression of platelet/endothelial cell adhesion molecule-1 （CD31）， vascular endothelial growth factor-α （VEGF-α）， vascular endothelial growth factor receptor 2 （VEGFR2）， and phosphorylated（p）-VEGFR2. Result:Compared with the NC group， the CIA group showed red and swollen ankle joints， increased arthritis index scores （P<0.05， P<0.01）， manifest injury in the knee and ankle joints， reduced cartilage thickness， elevated Micro CT bone destruction scores of knee and ankle joints （P<0.01）， and up-regulated absorbance values of synovial CD31， VEGF-α， VEGFR2， and p-VEGFR2 （P<0.01）. Compared with the CIA group， the DTYM group showed relieved ankle joint redness and swelling， reduced arthritis index scores of mice three weeks after administration （P<0.05， P<0.01）， intact joint surfaces of the knee and ankle joints， thickened cartilage， declining Micro CT bone destruction scores in both the knee and ankle joints （P<0.05， P<0.01）， and lowered absorbance values of CD31， VEGF-α， VEGFR2， and p-VEGFR2 in the synovium （P<0.01）. Conclusion:DTYM can inhibit the pannus formation in CIA mice presumedly by regulating the VEGF pathway.
