Effect of Buyang Huanwutang on Protein Expression of MAP-2， NF-M and GAP-43 After Sciatic Nerve Transection Anastomosis in Rats
10.13422/j.cnki.syfjx.20212039
- VernacularTitle:补阳还五汤对大鼠坐骨神经横断吻合术后MAP-2，NF-M，GAP-43蛋白表达的影响
- Author:
Si-cheng YAO
1
;
Xian-de MA
1
;
Lian-feng JIN
2
;
Chun-tian WANG
1
;
Chong-min CHEN
3
;
Xi-wei ZHANG
2
Author Information
1. Liaoning University of Traditional Chinese Medicine（TCM），Shenyang 110033，China
2. The Affiliated Hospital of Liaoning University of TCM，Shenyang 110033，China
3. Shenyang Orthopaedic Hospital，Shenyang 110044，China
- Publication Type:Research Article
- Keywords:
Buyang Huanwutang;
nerve transection anastomosis;
peripheral nerve regeneration;
nerve function;
sciatic nerve
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(20):23-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of Buyang Huanwutang on the expression of microtubule-associated protein-2（MAP-2）， neurofilament-M（NF-M）， and growth associated protein-43（GAP-43）in rat sciatic nerve after sciatic nerve transection and anastomosis. To explore the mechanism of Buyang Huanwutang promoting peripheral nerve regeneration. Method:SD rats were selected as the experimental subjects， and sciatic nerve transection model was selected as the experimental model. They were randomly divided into model group， sham operation group， Buyang Huanwutang group high， medium and low dose （29.6， 14.8， 7.4 g·kg-1）group， and mecobalamin （0.156 mg·kg-1）group， the model group and the sham operation group were given distilled water intragastric administration. After successful modeling， each group was treated with relevant drugs for 4 weeks. After 4 weeks， sciatic nerve function index（SFI）， degree of inclined plate test and hematoxylin-eosin（HE）of sciatic nerve in each group were tested. The expression levels of MAP-2， NF-M， and GAP-43 at the sciatic nerve anastomosis site were detected by immunohistochemistry and Western blot. Result:Compared with sham operation group， the expression levels of SFI， inclined plate test， MAP-2， NF-M and GAP-43 in model group were significantly increased （P<0.01）. Compared with model group， the expression levels of SFI， inclined plate test， MAP-2， NF-M and GAP-43 in Buyang Huanwutang high， medium and low-dose groups were significantly increased （P<0.05，P<0.01）. Conclusion:Buyang Huanwutang has a positive effect on nerve regeneration after sciatic nerve transection and anastomosis in rats.
