Mechanism of Duanteng Yimu Decoction on Human Umbilical Vein Endothelial Cell Model
10.13422/j.cnki.syfjx.20211706
- VernacularTitle:断藤益母汤对人脐静脉内皮细胞模型的作用机制
- Author:
Kai QIAN
1
;
Xue-xia ZHENG
1
;
Shu-di XU
1
;
Jing-yi ZHAN
1
;
Tang-ming YE
1
;
Fa-jie LIAO
1
;
Min-ying LIU
2
;
Chang-song LIN
2
Author Information
1. Guangzhou University of Chinese Medicine,Guangzhou 510405,China
2. The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510405,China
- Publication Type:Research Article
- Keywords:
Duanteng Yimu decoction;
human umbilical vein endothelial cells;
rheumatoid arthritis;
vascular endothelial growth factor (VEGF) signaling pathway;
endothelial cell activation;
angiogenesis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(19):36-45
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Duanteng Yimu decoction (DTYM) on the activation of the human umbilical vein endothelial cell (HUVEC) model and the effect on related activated proteins and vascular endothelial growth factor (VEGF) signaling pathway. Method:After DTYM (200, 400 g·mL-1) treatment of HUVEC induced by VEGF and tumor necrosis factor-α (TNF-α), cell proliferation, migration, and tubulogenesis were detected by cell counting kit-8 (CCK-8) assay, 5-ethynyl-2'-deoxyuridine (EdU) assay, transwell migration assay, phalloidin staining, and matrix gel card method. The mRNA expression of adhesion factors, including E-selectin, intercellular adhesion molecule-1 (ICAM-1), and vascular cell adhesion molecule-1 (VCAM-1) was detected by real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). The expression of von Willebrand factor (VWF), platelet-endothelial cell adhesion molecule-31 (CD31), angiogenic factor cysteine-rich-61 (CYR61), angiopoietin-1 (ANG-1), VEGF, and VEGF receptor-2 (VEGFR2) was detected by Western blot. Immunofluorescence was used to determine CD31 expression. Result:Compared with the normal group, the model group showed potentiated proliferation, migration, and tubulogenesis of HUVEC (P<0.05, P<0.01), elevated mRNA expression of E-selectin, ICAM-1, and VCAM-1 (P<0.01), up-regulated protein expression of VWF, CD31, ANG-1, CYR61, VEGF-α, and phospho (p)-VEGFR2 (P<0.05, P<0.01), and increased CD31 immunofluorescence intensity (P<0.01). Compared with the model group, the DTYM groups displayed blunted proliferation, migration, and tubulogenesis of HUVEC (P<0.05, P<0.01), decreased mRNA expression of E-selectin, ICAM-1, and VCAM-1 (P<0.05, P<0.01), down-regulated protein expression of VWF, CD31, ANG-1, CYR61, VEGF-α, and p-VEGFR2 (P<0.05, P<0.01), and weakened CD31 immunofluorescence intensity (P<0.01). Conclusion:DTYM inhibits HUVEC proliferation, migration, adhesion, and tubulogenesis, which is associated with the regulation of CD31, VWF, CYR61, and ANG-1 expression in HUVEC and the VEGF signaling pathway.