Chronic Toxicity of Tanreqing Injection in Beagle Dogs
10.13422/j.cnki.syfjx.20211894
- VernacularTitle:痰热清注射液对Beagle犬的长期毒性实验
- Author:
Fan WANG
1
;
Fa-kai LU
2
;
Lian-zhong ZHANG
3
;
Xue-hang DU
2
;
Xi-jie DU
2
;
Xiao-li ZHANG
2
Author Information
1. Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine，Shanghai 201203， China
2. Shanghai Kai Bao Pharmaceutical Co.， Ltd.， Shanghai 201401， China
3. Center for Disease Control and Prevention， Pingchuan District， Baiyin city， Gansu 730913， China
- Publication Type:Research Article
- Keywords:
Tanreqing injection;
long-term toxicity;
Beagle dogs;
fibrinogen;
Kupffer's cells
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(18):73-79
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the possible toxicity of long-term intravenous injection of Tanreqing injection in Beagle dogs， so as to provide experimental data for its clinical safe medication. Method:A total of 32 Beagle dogs （16 males and 16 females） were randomly divided into the low- （2.5 mL·kg-1）， medium- （5.0 mL·kg-1）， and high-dose （10.0 mL·kg-1） Tanreqing injection groups and control group according to their body mass indices， with eight dogs in each group. In the waking state， the dogs were treated with intravenous injection of corresponding drugs into the medial cephalic vein of forelimb for 13 weeks， followed by four-week drug withdrawal. After the observation of general condition， body mass， and food consumption， the Beagle dogs were subjected to electrocardiography， ophthalmoscopy， hematological examination， serum biochemistry， and blood coagulation test in the middle of medication （week 6）， at the end of medication （week 13）， and during recovery （week 17）. Then the gross anatomy was conducted for calculating the major organ coefficients and observing the histopathological changes. Result:No obvious toxic reaction was found in each group， but the decreased fibrinogen and increased Kupffer's cells phagocytizing yellow-brown pigment in hepatic sinusoids were observed in the high-dose Tanreqing injection group following three months of medication. Reduction of fibrinogen was not observed in recovery period， but Kupffer's cells that phagocytized yellow-brown pigment still existed. Conclusion:The intravenous injection of Tanreqing injection at 2.50 mL·kg-1 （low dose）， 5.00 mL·kg-1 （medium dose） or 10.00 mL·kg-1 （high dose） for three months in Beagle dogs resulted in no obvious toxic reaction. However， it is still suggested to test the liver function and blood coagulation after long-term administration of high-dose Tanreqing injection.
