Effect of Different Extracts of Thlaspi Herba on Gut Microbiota of Hyperuricemia Mice
10.13422/j.cnki.syfjx.20211249
- VernacularTitle:菥蓂不同提取物对高尿酸血症小鼠肠道菌群的影响
- Author:
Xi KANG
1
;
Hui-hui HAO
1
;
Cheng-lin MU
2
;
Tao-tao LU
2
;
Xue-lian WANG
2
;
Li-jing TANG
2
;
Rong-ping YANG
2
Author Information
1. School of Pharmacy，Chengdu University of Traditional Chinese Medicine，Chengdu 611137，China
2. College of Pharmaceutical Sciences， Southwest University，Chongqing 400715，China
- Publication Type:Research Article
- Keywords:
Thlaspi Herba;
hyperuricemia;
gut microbiota;
xanthine oxidase （XOD）;
flavonoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):132-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of different extracts of Thlaspi Herba on the gut microbiota of hyperuricemia mice， and to reveal the substance basis and mechanism of its hypouricemic activity. Method:Eighty-eight male Kunming mice were divided into 11 groups， including blank group， model group， allopurinol group， high and low dose groups of petroleum ether extract， high and low dose groups of ethyl acetate extract， high and low dose groups of n-butanol extract， high and low dose groups of total flavonoids extract. Mice in the blank group were given 0.5% sodium carboxymethylcellulose by gavage， and the other groups were given oteracil potassium （500 mg·kg-1） by gavage to duplicate the hyperuricemia model. After modeling for several hours， the blank group and the model group were given distilled water by gavage， while mice in the allopurinol group were given allopurinol suspension （50 mg·kg-1）， and mice in each treatment group were given high and low doses of corresponding extract （5， 2.5 g·kg-1）. The serum uric acid （SUA） level and xanthine oxidase （XOD） activity were measured after 14 days. Fresh feces were collected for 16S rDNA sequencing. Result:Compared with the blank group， SUA level and XOD activity of model group were significantly increased （P<0.05）. Compared with the model group， SUA level and XOD activity of the allopurinol group were significantly decreased （P<0.01）. After intervention， SUA level were significantly decreased （P<0.05， P<0.01）， except for high dose and low dose groups of petroleum ether extract and low dose group of total flavonoids extract， XOD activity was significantly inhibited in low dose group of petroleum ether extract， high dose group of total flavonoids extract， and high and low dose groups of n-butanol extract （P<0.05， P<0.01）. The high dose group of total flavonoids extract was the most significant. The results of flora sequencing showed that α diversity and abundance of the model group changed significantly， and Bacteroidetes， Firmicutes and Lactobacillaceae were significantly correlated with XOD activity. After intervention， the operational taxonomic unit （OTU）， ACE， Chao1 and Shannon indexes of the high and low dose groups of total flavonoids extract were significantly increased （P<0.05， P<0.01）. Relative abundance of Bacteroidetes in low dose group of ethyl acetate extract， high dose group of total flavonoids extract， and high and low dose groups of n-butanol extract was significantly decreased （P<0.01）， and the relative abundance of Firmicutes was significantly increased （P<0.01）. The relative abundance of Lactobacillaceae in low dose group of n-butanol extract and high dose group of total flavonoids extract was significantly increased （P<0.01）. Conclusion:The effective part of Thlaspi Herba for reducing uric acid is mainly flavonoids， the improvement of SUA level and XOD activity by affecting gut microbiota such as Lactobacillaceae， Bacteroidetes and Firmicutes， may be one of its mechanisms.
