Difference Analysis of Pharmacokinetic Behavior of Active Ingredients in Wujiwan Between Normal Rats and Rats with Irritable Bowel Syndrome
10.13422/j.cnki.syfjx.20211048
- VernacularTitle:戊己丸中活性成分在正常大鼠和肠易激综合征大鼠体内药代动力学行为的差异性分析
- Author:
Hong ZHANG
1
;
Ying CHEN
2
;
Zi-peng GONG
2
;
Yu DONG
3
;
Zi-yu YANG
2
;
Qing YANG
2
;
Yu-jie LI
2
;
Wei-yan CAI
2
;
Xiao-gang WENG
2
;
Ya-jie WANG
2
;
Qi LI
2
;
Yuan-long ZANG
1
;
Xiao-xin ZHU
1
Author Information
1. Anhui University of Chinese Medicine，Hefei 230038，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
3. Guang'anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 100053，China
- Publication Type:Research Article
- Keywords:
Wujiwan;
irritable bowel syndrome （IBS）;
ultra-performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS）;
neonatal rat colon balloon stimulation method;
pharmacokinetics;
chronic visceral hypersensitivity;
alkaloids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):124-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an ultra-performance liquid chromatography-mass spectrometry （UPLC-MS/MS） for determining the plasma concentrations of 10 active ingredients in Wujiwan at different time points after oral administration， and to compare the pharmacokinetic characteristics between normal rats and rats with chronic visceral hypersensitive irritable bowel syndrome （CVH-IBS）. Method:CVH-IBS rat model was prepared by the neonatal rat colon percutaneous transluminal coronary angioplasty （PTCA） balloon stimulation method. After intragastric administration of Wujiwan （0.245 g·kg-1）， blood was collected from the jugular vein at different time points， and the plasma concentrations of 10 active ingredients （berberine hydrochloride， palmatine hydrochloride， coptisine hydrochloride， jatrorrhizine hydrochloride， epiberberine， dihydroberberine， evodiamine， evodine， paeoniflorin， albiflorin） in Wujiwan was detected simultaneously by UPLC-MS/MS， the pharmacokinetic parameters of each component in normal rats and CVH-IBS rats were calculated. Result:The established UPLC-MS/MS could sensitively and accurately detect the plasma concentrations of 10 active ingredients of Wujiwan in rats. Compared with the normal group， the absorption rates of these 10 active ingredients of Wujiwan in the blood of CVH-IBS rats all decreased to a certain extent， and the peak time （tmax） was prolonged. Among them， the tmax of berberine hydrochloride and jatrorrhizine hydrochloride were significantly prolonged from 54 minute and 39 minute to 90 minute， respectively （P<0.05， P<0.01）. Area under the plasma concentration-time curve （AUC0-t） of each component increased， and evodiamine and paeoniflorin were significantly different （P<0.05， P<0.01）. The clearance rates （CL/F） of these 10 active ingredients were all decreased， among which berberine hydrochloride， palmatine hydrochloride and evodiamine had significant differences （P<0.05， P<0.01）. Conclusion:There are significant differences in the pharmacokinetic behavior of the active ingredients in Wujiwan between normal rats and CVH-IBS rats， which may be related to the destruction of microstructure of intestinal epithelial cells and the change of activity of liver enzymes under the pathological state of IBS.
