Effect of Danggui Shaoyaosan on TLR4/MyD88/JNK Signaling Pathway on Rats with Nonalcoholic Fatty Liver Disease
10.13422/j.cnki.syfjx.20211737
- VernacularTitle:当归芍药散对非酒精性脂肪肝大鼠TLR4/MyD88/JNK信号通路的影响
- Author:
Yi-shui LI
1
;
Xin-qiao CHU
2
;
Ya-ning BIAO
1
;
Mu-qing ZHANG
3
;
Yi-lu HAN
1
;
Zi-xuan LI
1
;
Yi-xin ZHANG
1
Author Information
1. School of Pharmacy， Application Technology Research Center of Traditional Chinese Medicine （TCM）Prescription in University of Hebei Province，Hebei University of Chinese Medicine，Shijiazhuang 050200，China
2. Guang 'anmen Hospital，China Academy of Chinese Medical Sciences，Beijing 100053，China
3. Hebei Provincial Hospital of TCM，Shijiazhuang 050911，China
- Publication Type:Research Article
- Keywords:
Danggui Shaoyaosan;
nonalcoholic fatty liver disease;
Toll-like receptor 4 （TLR4）;
myeloid different factory 88 （MyD88）;
c-Jun N-terminal kinase （JNK）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(17):24-31
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the efficacy and mechanism of Danggui Shaoyaosan on rats of nonalcoholic fatty liver disease （NAFLD）. Method:Sixrty SPF SD male rats were randomly divided into normal group， model group，essentiale （0.144 g·kg-1） and low， middle and high-dose of Danggui Shaoyaosan groups （2.44， 4.88， 9.76 g·kg-1）. High fat diet were fed to bulid the NAFLD model， and each treatment group was given corresponding drugs at the same time. After 8 weeks， the serum and liver tissue were collected to detect the contents or activities of total cholesterol （TC）， triglyceride （TG）， alanine aminotransferase （ALT）， aspartic acid aminotransferase （AST）， tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） in serum， the contents of TC， TG and free fatty acid （FFA） in liver tissue， Real-time fluorescence quantitative polymerase chain reaction（Real-time PCR） and Western blot were used to observe the gene and protein expressions of Toll-like receptor 4 （TLR4）， myeloid different factory 88 （MyD88） and c-Jun n-terminal kinase （JNK） and the protein expression of phosphorylation JNK（p-JNK） in liver tissue. Hematoxylin-eosin （HE） staining and Oil red staining to observe the pathological morphological changes of liver. Result:Compared with control group， the contents or activities of TC， TG， ALT， AST and TNF-α in serum， the contents of TC， TG and FFA in liver and the gene and protein expressions of TLR4， MyD88， JNK， and the protein expression of p-JNK in liver tissue of model group were distinctly increased （P<0.01）， the content of IL-10 was significantly decreased （P<0.01）. Compared with model group， the contents or activities of TC， TG， ALT， AST and TNF-α in serum， the contents of TC， TG and FFA in liver and the mRNA and protein expressions of TLR4， MyD88 and JNK， and the protein expression of p-JNK in liver tissue of Danggui Shaoyaosan groups were significantly reduced （P<0.05，P<0.01）， the content of IL-10 in serum of Danggui Shaoyaosan groups was distinctly increased（P<0.05，P<0.01）， HE staining and Oil red staining show that the degree of liver steatosis was alleviated obviously by Danggui Shaoyaosan. Conclusion:Danggui Shaoyaosan has a better treatment on NAFLD by inhibiting TLR4/MyD88/JNK pathway and alleviating the inflammation response.
