Quality Evaluation of Citri Sarcodactylis Fructus from Different Origins Based on HPLC Fingerprint and Chemometrics
10.13422/j.cnki.syfjx.20211551
- VernacularTitle:基于HPLC指纹图谱结合化学计量学评价不同产地佛手药材质量
- Author:
Zhen-xing ZHENG
1
;
Han-wen HU
2
;
Li ZENG
1
;
Huan YANG
1
;
Yu-xin FAN
1
;
Da-le GUO
1
;
Fang DENG
1
Author Information
1. School of Pharmacy， Chengdu University of Traditional Chinese Medicine（TCM）， State Key Laboratory of Southwestern Chinese Medicine Resources， Key Laboratory of Standardization of Chinese Herbal Medicine，Ministry of Education， Chengdu 611137，China
2. Yuechi Hospital of TCM，Guang'an 638300，China
- Publication Type:Research Article
- Keywords:
Citri Sarcodactylis Fructus;
high performance liquid chromatography （HPLC） fingerprint;
chemometrics;
cluster analysis;
principal component analysis （PCA）;
orthogonal partial least squares discriminant analysis （OPLS-DA）;
quality evaluation
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(21):174-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the high performance liquid chromatography （HPLC） fingerprint of Citri Sarcodactylis Fructus， and to search for makers to characterize the quality difference of Citri Sarcodactylis Fructus from different origins coupled with chemometrics. Method:The analysis was performed on a Thermo Hypersil GOLD C18 column （4.6 mm×250 mm， 5 μm） with mobile phase consisted of acetonitrile-0.05% phosphoric acid solution for gradient elution， and the detection wavelength was set at 254 nm. A total of 31 batches of samples were analyzed to establish the HPLC fingerprint of Citri Sarcodactylis Fructus. Similarity evaluation was performed by Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System （2012 edition） to confirm the common peaks， which were identified by comparison of reference substances. On the basis， chemometrics methods were used to analyze and evaluate the quality of Citri Sarcodactylis Fructus from different origins. At the same time， 3 batches of 5 species of decoction pieces from the genus Citrus in the family Rutaceae， including Citri Sarcodactylis Fructus， Aurantii Fructus Immaturus， Aurantii Fructus， Citri Reticulatae Pericarpium Viride and Citri Reticulatae Pericarpium， were randomly collected for evaluating the effectiveness and reliability of the established HPLC fingerprint of Citri Sarcodactylis Fructus. Result:HPLC fingerprint of Citri Sarcodactylis Fructus was established and 22 common peaks were identified. And seven common peaks among them were identified as 6，7-dimethoxycoumarin， diosmin， hesperidin， byakangelicin， 5，7-dimethoxycoumarin， bergapten and oxypeucedanin. Except for 2 batches of samples， the similarities of fingerprints between other 29 batches of samples were >0.9. The 31 batches of Citri Sarcodactylis Fructus were basically divided into 3 groups by cluster analysis and principal component analysis， which were consistent with the classification of three different producing areas. Eight differential markers were screened by orthogonal partial least squares discriminant analysis and four of them （5，7-dimethoxycoumarin， bergapten， 6，7-dimethoxycoumarin and diosmin） were identified by reference substances. Similarity evaluation of 5 species of decoction pieces from genus Citrus in the family Rutaceae was carried out by taking the reference fingerprint of Citri Sarcodactylis Fructus as treference chromatogram， similarity of Citri Sarcodactylis Fructus decoction pieces was 0.892-0.977， and the similarities of the other 4 kinds of decoction pieces were 0.215-0.517. Conclusion:The established fingerprint method is reasonable， effective and accurate for quality control of Citri Sarcodactylis Fructus， the characterization information is more comprehensive combined with chemometrics.
