Identification and Attribution of Chemical Constituents in Naizilai Granules by UHPLC-Q-Orbitrap-MS
10.13422/j.cnki.syfjx.20211647
- VernacularTitle:乃孜来颗粒中化学成分的UHPLC-Q-Orbitrap-MS分析
- Author:
Ya-hui YAN
1
;
Tao WU
2
;
Ju CHEN
3
;
Rahima ABDULA
2
;
Lei HUANG
3
;
Haji-akber AISA
1
Author Information
1. University of Chinese Academy of Sciences，Beijing 100049，China
2. Key Laboratory of Plant Resources and Chemistry in Arid Regions， Xinjiang Technical Institute of Physics and Chemistry，Chinese Academy of Sciences， Urumqi 830011，China
3. Xinjiang Yinduolan Uighur Medcine Co. Ltd.，Urumqi 830011，China
- Publication Type:Research Article
- Keywords:
Naizilai granules;
ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry （UHPLC-Q-Orbitrap-MS）;
flavonoids;
organic acids;
sesquiterpenoids;
chemical constituents;
coumarins
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(21):156-166
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Ultra-high performance liquid chromatography-quadrupole/orbitrap high resolution mass spectrometry （UHPLC-Q-Orbitrap-MS） was used to rapidly analyze and assign the chemical constituents of Naizilai granules. Method:An ACQUITY UPLC BEH Shield RP C18 column （2.1 mm×100 mm， 1.7 µm） was selected for chromatographic analysis， the mobile phase was 0.1% formic acid aqueous solution （A） and acetonitrile （B） for gradient elution （0-3 min， 1%B； 3-16 min， 1%-11%B； 16-30 min， 11%-34%B； 30-37 min， 34%-52%B； 37-42 min， 52%-100%B； 42-44 min， 100%B）， flow rate was 0.3 mL·min-1 and the column temperature was 35 ℃. Mass spectrometry data of Naizilai granules were collected in positive and negative ion modes， the chemical constituents of this preparation were speculated and identified according to the precise molecular weight， secondary fragmentation and other information， combined with reference substance and literature data. Result:A total of 175 compounds were identified and speculated， including 72 flavonoids， 77 organic acids， 15 sesquiterpenes， 6 coumarins and 5 other compounds. Among these identified chemical constituents， there were 154 from Artemisia rupestris， 64 from Hyssopus cuspidatus， 33 from Cordia dichotoma， 42 from Viola tianshanica， 56 from Lactuca sativa， 65 from Mentha haplocalyx， 78 from Matricaria chamomilla， 28 from Ziziphus jujuba， 7 of which were common components of these eight herbs. Conclusion:The established analytical method can realize the rapid and accurate identification of the chemical constituents in Naizilai granules， and basically covers the main constituents of each medicinal material in the formula， so as to provide a basis for improving the quality evaluation system of the preparation and lay a foundation for elucidating the pharmacodynamic mechanism.
