Effect of Danggui Shaoyaosan on Diminished Ovarian Reserve in Model Rats： An Exploration Based on TGF-β1/ Smads Signaling Pathway
10.13422/j.cnki.syfjx.20212004
- VernacularTitle:基于TGF-β1/Smads信号通路探讨当归芍药散对卵巢储备功能低下模型大鼠的影响
- Author:
Ya-ping ZHANG
1
;
Chen YOU
1
;
Yu-na WEI
1
;
Shan-shan JIANG
2
;
Ya-jing WANG
2
;
Qiao-yan PANG
2
Author Information
1. Basic Medical College of Guangxi University of Chinese Medicine，Nanning 530000，China
2. Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine，Nanning 530000，China
- Publication Type:Research Article
- Keywords:
Danggui Shaoyaosan;
diminished ovarian reserve （DOR）;
stress;
transforming growth factor-β1 （TGF-β1）/Smads signaling pathway
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(21):47-54
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the improving effect of Danggui Shaoyaosan on diminished ovarian reserve （DOR） in rats triggered by Tripterygia wilfordii polyglycoside tablet combined with stress， and to explore the role of transforming growth factor-β1 （TGF-β1）/Smads signaling pathway in such improvement. Method:Forty-eight female SD rats with normal sexual cycle were selected and randomly divided into a normal group （n=8） and a modeling group （n=40）， and the ones in the modeling group were given Tripterygium wilfordii polyglycoside tablets （50 mg·kg-1） combined with random stress for 15 d. After successful modeling， the rats were randomized into the model group， low-， medium-， and high-dose （3.96， 7.92， 15.84 g·kg-1） Danggui Shaoyaosan groups， and estradiol valerate group （0.09 mg·kg-1）， with eight in each group. Under the premise of stress exposure， they were separately gavaged with the normal saline， low-， medium- and high-dose Danggui Shaoyaosan， and estradiol valerate for 15 successive days. The estrous cycle of rats in each group was observed daily. After intervention， the rats were sacrificed and the ovarian visceral index was calculated. The pathological changes in ovarian tissues were observed by hematoxylin eosin （HE） staining. The protein expression levels of TGF-β1 and TGF-β1 receptor （TGF-β1R） in the ovarian tissues of rats were measured by immunohistochemistry （IHC）， and the mRNA expression levels of Smad2， Smad3， and Smad7 in the ovarian tissues by real-time polymerase chain reaction （Real-time PCR）. Result:Compared with the normal group， the model group exhibited disordered estrus cycle （P<0.05）， reduced visceral index （P<0.01）， and down-regulated TGF-β1 and TGF-β1R protein and Smad2 and Smad3 mRNA expression in the ovarian tissues （P<0.01）， and up-regulated Smad7 mRNA expression （P<0.01）. Compared with the model group， Danggui Shaoyaosan at the low， medium， and high doses and estradiol valerate improved the estrus cycle of rats to varying degrees （P<0.05） and increased the visceral index， with better effects observed in the medium-group and high-dose Danggui Shaoyaosan groups （P<0.05，P<0.01）. Besides， the protein expression levels of TGF-β1 and TGF-β1R and the mRNA expression levels of Smad2 and Smad3 in the ovarian tissues were elevated to varying degrees （P<0.01）， and the Smad7 mRNA expression declined （P<0.01）. The improvements in TGF-β1 and TGF-β1R protein expression of the medium-dose Danggui Shaoyaosan group and estradiol valerate group were more obvious. Conclusion:Danggui Shaoyaosan significantly improves ovarian reserve in DOR rats， which is closely related to the regulation of TGF-β1/Smads signaling pathway.
