Co-Treatment of Phlegm and Stasis Improves Myocardial Inflammation in Rats with Diabetes Mellitus via TLR4/NF-κB/IκB Pathway
10.13422/j.cnki.syfjx.20212237
- VernacularTitle:痰瘀同治调控TLR4/NF-κB/IκB通路对糖尿病大鼠心肌炎症反应的影响
- Author:
Fei-xiang LI
1
;
Quan-gen CHU
1
;
Pan WANG
1
;
Jin YU
1
;
Jun CHU
2
Author Information
1. College of Traditional Chinese Medicine， Anhui University of Chinese Medicine， Hefei 230038， China
2. Key Laboratory of Xin'an Medicine， Ministry of Education， Anhui University of Chinese Medicine， Hefei 230038， China
- Publication Type:Research Article
- Keywords:
diabetic cardiomyopathy;
co-treatment of phlegm and stasis;
Didang Xianxiongtang;
Toll-like receptor 4 （TLR4）/nuclear factor-κB （NF-κB）/NF-κB inhibitor （IκB） signaling pathway;
inflammatory response
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(22):59-64
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the intervention of phlegm-stasis co-treatment on the myocardial Toll-like receptor 4 （TLR4）/nuclear factor-κB （NF-κB）/nuclear factor-κB inhibitor （IκB） signaling pathway， and to investigate its mechanism in improving myocardial inflammation in rats with diabetes mellitus （DM）. Method:Forty-five male SD rats of SPF grade were randomly divided into a normal group， a phlegm-resolving （Xiao Xianxiongtang， 4.05 g·kg-1） group， a stasis-resolving （Xuefu Zhuyutang， 7.02 g·kg-1） group， a co-treatment （Didang Xianxiong decoction， 8.10 g·kg-1） group， an alagebrium chloride （3 mg·kg-1） group， and a model group. Except for normal group， the other rats was induced by a single intraperitoneal injection of 55 mg·kg-1 streptozotocin （STZ） to establish DM model. After adaptive feeding for three weeks， the rats were treated correspondingly by gavage daily for eight weeks. Rats were sampled under anesthesia. Enzyme-linked immunosorbent assay（ELISA） was used to detect the protein expression of TLR4 and tumor necrosis factor-alpha （TNF-α） in myocardial tissues. The expression levels of NF-κB p65 and IκBα were detected by immunohistochemistry. NF-κB p65， IκBα， TNF-α， and TLR4 mRNA expression levels were detected by real-time fluorescence-based quantitative polymerase chain reaction（Real-time PCR）. Result:The protein and mRNA levels of TLR4， NF-κB p65， IκBα， and TNF-α were higher in the model group than those in the normal group （P<0.01）. TLR4， NF-κB p65， IκBα， and TNF-α protein and mRNA expression levels were reduced to varying degrees in the groups with drug intervention as compared with those in the model group （P<0.01）. The inter-group comparison revealed that the co-treatment group showed more manifest reduction in protein and mRNA expression levels of TLR4， NF-κB p65， IκBα， and TNF-α than the phlegm-resolving group and the stasis-resolving group （P<0.05，P<0.01）. Conclusion:The co-treatment of phlegm and stasis can improve myocardial inflammation in DM rats， with superior effect to either the phlegm-resolving method or the stasis-resolving method. The underlying mechanism may be related to the inhibition of TLR4/NF-κB/IκB signaling pathway activation.
