Effect of Panlongqi Tablet on Cervical Spondylosis of Vertebral Artery Type in Rats
10.13422/j.cnki.syfjx.20212238
- VernacularTitle:盘龙七片对椎动脉型颈椎病大鼠的干预作用
- Author:
Rui-rui MING
1
;
Teng-teng XU
2
;
Jin-xia WANG
2
;
Ke-xin JIA
2
;
Chun-fang LIU
2
;
Wei-heng CHEN
3
;
Na LIN
1
Author Information
1. Chengde Medical University，Chengde 067000，China
2. Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences，Beijing 100700，China
3. The Third Affiliated Hospital of Beijing University of Chinese Medicine， Beijing 100029， China
- Publication Type:Research Article
- Keywords:
Panlongqi pill;
rats;
cervical spondylosis of vertebral artery type;
vertebral artery blood flow;
vasomotor function
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(22):51-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a model of cervical spondylosis of vertebral artery type （CSA） in rats by mixed modeling method， and observe the intervention effect of Panlongqi tablet （PLQT） on CSA rats. Method:SD rats were divided into a normal control group， a model group， low- （0.16 g·kg-1）， medium- （0.32 g·kg-1）， and high-dose （0.64 g·kg-1） PLQT groups， and a Jingfukang granule （JFK， 1.35 g·kg-1） group. The rats were treated correspondingly 24 hours after modeling for eight weeks， and those in the normal control group received an equal volume of normal saline by gavage. The limb movement was tested by the inclined plate assay， vertebral artery flow volume by multi-mode high-frequency sound wave for small animals， and microcirculatory blood flow in the pia mater by the laser Doppler. The imaging of the cervical spine was recorded and scored by X-ray micro-computed tomography （Micro CT）. Enzyme-linked immunosorbent assay （ELISA） was used to detect serum levels of endothelin-1 （ET-1）， nitric oxide （NO）， tissue plasminogen activator （t-PA）， and plasminogen activator inhibitor （PAI）. Result:Compared with the normal control group， the model group showed decreased limb movement， vertebral artery flow volume， and microcirculatory blood flow in the pia mater， and increased imaging of the cervical spine and score （P<0.05，P<0.01）. PLQT could dose-dependently improve the motor function， increase the vertebral artery flow volume and microcirculatory blood flow in the pia mater， and reduce the degree and score of imaging of the cervical spine in CSA rats（P<0.05，P<0.01）. The serum levels of NO and t-PA were decreased and those of ET-1 and PAI were increased in the model group as compared with those in the normal control group， while such changes were reversed by PLQT treatment（P<0.05，P<0.01）. Conclusion:PLQT can enhance the limb movement， promote the vertebral artery flow volume and microcirculatory blood flow in the pia mater， improve the degree of imaging of the cervical spine， regulate the vasomotor function， and improve the coagulation and fibrinolysis system of CSA rats， which shows good potential for the treatment of CSA.
