Isolation of Growth-Promoting Bacteria and Effect of Compound Bacteria on Yield of Fritillaria przewalskii
10.13422/j.cnki.syfjx.20211512
- VernacularTitle:促生细菌的分离及复配菌剂对甘肃贝母产量的影响
- Author:
Jiang ZHAO
1
;
Shi-jun LIANG
2
;
Tao YANG
1
;
Xiao-long WU
2
;
Peng-nian QI
3
;
Shi-wei WANG
3
;
Zhi-ye WANG
1
Author Information
1. Institute of Biology，Gansu Academy of Sciences，Lanzhou 730000，China
2. Huating Traditional Chinese Medicine Industry Development Service Center，Huating 744100，China
3. Gansu Lvneng Agricultural Technology Co. Ltd.，Wuwei 733000，China
- Publication Type:Research Article
- Keywords:
Fritillaria przewalskii;
Bacillus;
Pseudomonas;
growth-promoting effect
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):163-170
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of anti-microbial compound （T1） from Bacillus （Phylum Firmicutes） and anti-microbial compound （T2） from Pseudomonas and Rhizobium， two growth-promoting agents， on the physiological characteristics and growth of Fritillaria przewalskii， in order to lay a foundation for the development of functional microbial agents and the promotion of ecological planting. Method:The endophytic bacteria of F. przewalskii were isolated and identified using conventional methods. The leaves of three-year-old F. przewalskii were sprayed with T1 and T2， followed by yield determination. The enzyme activities and physiological and biochemical indexes in the plant and microorganisms were measured using the corresponding assay kits， and the contents of related hormones by liquid chromatography-mass spectrometry （LC-MS）. Result:The isolated endophytic bacteria were classified into Firmicutes，Proteobacteria， and Actinomycetes. The activities of superoxide dismutase（SOD） and peroxidase（POD） and auxin content after T2 treatment were significantly higher than those after T1 treatment， while the contents of siderophore，salicylic acid， and gibberellin were lower. Compared with the blank （CK） group， T1 and T2 increased the contents of endogenous gibberellin，cytokinin， and auxin in F. przewalskii leaves，but did not significantly change jasmonic acid and abscisic acid. T1 promoted the accumulation of endogenous salicylic acid in F. przewalskii leaves， but there was no significant change after T2 treatment. Compared with CK，T1 and T2 enhanced the activities of SOD， POD， and catalase （CAT） and decreased the content of malondialdehyde. T2 promoted the accumulation of hydrogen peroxide in F. przewalskii leaves， but no significant difference was observed after T1 treatment. Compared with CK，both T1 and T2 increased chlorophyll，average iron content in rhizosphere soil， and 100-plant weight. Conclusion:T1 and T2 treatments help to increase the yield，and their specific mechanisms differ from each other. T1 exhibits better effect than T2.
