Development of Fucosterol Certified Reference Material
10.13422/j.cnki.syfjx.20211771
- VernacularTitle:岩藻甾醇标准样品的研制
- Author:
Wen-hui JIN
1
;
Yi-ping ZHANG
1
;
Wei-zhu CHEN
1
;
Hua FANG
1
;
Zhuan HONG
1
;
Zhi-mao CHAO
2
Author Information
1. Technology Innovation Center for Exploitation and Utilization of Marine Biological Resources， Third Institute of Oceanography，Ministry of Natural Resources，Xiamen 361005，China
2. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
- Publication Type:Research Article
- Keywords:
fucosterol;
certified reference material;
homogeneity;
stability;
certification;
Laminaria japonica dried thallus;
high performance liquid chromatography （HPLC）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):131-137
- CountryChina
- Language:Chinese
-
Abstract:
Objective:According to the GB/T 15000.3-2008， to develop a fucosterol certified reference material based on the project approved by Standardization Administration. Method:Fucosterol was isolated from Laminaria japonica dried thallus via 95% ethanol extraction， vacuum concentration， repeated column chromatography separation， recrystallization in petroleum ether-ethyl acetate， and residual solvent removal. Its chemical structure was identified by elemental analysis （EA）， infrared spectrum （IR）， mass spectrometry （MS）， nuclear magnetic resonance （NMR） and X-ray diffraction （XRD）. Its homogeneity， stability， and cooperative certification conducted by 8 laboratories were carried out by high performance liquid chromatography with evaporative light scattering detector. Result:For the fucosterol reference material， the certified value of purity was 99.54% with expanded uncertainty of 0.16% in confidence interval of 95%， the stability was good within 24 months storage period at 2-4 ℃， which met the technical requirements of reference material and passed the acceptance organized by Standardization Administration. Conclusion:The national standard materials of fucosterol has been successfully developed， which can be used for the determination of this component， the evaluation of detection methods， and the detection and quality control of related products.
