Effect of Huoxue Jiedu Runzao Prescription on Expression of Apoptosis Inhibitor Survivin in Submandibular Gland Cells of Sjögren's Syndrome Mice
10.13422/j.cnki.syfjx.20212438
- VernacularTitle:活血解毒润燥方对干燥综合征小鼠颌下腺细胞凋亡抑制因子Survivin表达的影响
- Author:
Fang-kai LI
1
;
Yue-lan ZHU
1
;
Xiu-juan HOU
1
;
Yu-ting ZHANG
1
;
Xin-yi GU
2
;
Hai-bo WANG
1
;
Qian SHEN
1
Author Information
1. Dongfang Hospital of Beijing University of Traditional Chinese Medicine， Beijing 100078， China
2. Tongzhou Branch of Dongzhimen Hospital of Beijing University of Chinese Medicine， Beijing 101121， China
- Publication Type:Research Article
- Keywords:
Sjögren's syndrome （SS）;
submandibular gland;
apoptosis;
Survivin;
Huoxue Jiedu Runzao prescription
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):84-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Huoxue Jiedu Runzao prescription on the morphology， apoptosis， and function of submandibular gland in the mouse model of Sjögren's syndrome （SS） and its functioning mechanism， we analyzed the expression of the apoptosis inhibitor Survivin in the submandibular gland cells of SS mice. Method:Female BALB/c57 mice were selected as the normal group. The naive non-obese diabetic （NOD/Ltj） female mice were selected as the SS model， which were randomly assigned into the model group， Paeoniae Radix Alba total glucosides capsule （0.234 g·kg-1） group， and low-， medium-， and high-dose （15.6， 31.2， 62.4 g·kg-1， respectively） Huoxue Jiedu Runzao prescription groups. Each group had 15 mice. The morphological and functional changes of submandibular gland and the Survivin expression were observed and measured after 8 weeks of drug intervention. Survivin expression was determined by immunohistochemistry （IHC）， Western blot， and reverse transcription-polymerase chain reaction （RT-PCR）. Result:Compared with normal group， salivary flow and submandibular gland index in model group were significantly decreased （P<0.01）， and histopathological score of submandibular gland was significantly increased （P<0.01）. Western blot showed that Survivin protein expression was significantly decreased （P<0.05）. IHC showed that， Survivin mRNA expression was significantly decreased （P<0.01）， and RT-PCR results showed that Survivin mRNA expression was significantly decreased （P<0.01）. Compared with model group， salivary flow and submandibular gland index of mice in Huoxue Jiudu Runzao prescription groups and Paeoniae Radix Alba total glucosides capsules groups were significantly increased （P<0.05）， histopathological score of submandibular gland was significantly decreased （P<0.05）， IHC results showed that Survivin expression was significantly increased （P<0.01）. Western blot and RT-PCR results showed that Survivin protein and mRNA expression of Huoxue Jiudu Runzao prescription high-dose group and Paeoniae Radix Alba total glucosides capsule group were significantly increased （P<0.05）. Conclusion:Huoxue Jiedu Runzao prescription can improve the secretion function of submandibular acinus， increase the submandibular gland index， and saliva secretion of SS mice by up-regulating survivin in submandibular gland cells of SS mice.
