Inhibitory Effect and Mechanism of Jingulian Capsule on Human Breast Cancer MDA-MB-231 Cells
10.13422/j.cnki.syfjx.20212424
- VernacularTitle:金骨莲胶囊对人乳腺癌MDA-MB-231细胞的抑制作用及机制
- Author:
Jian-fei QIU
1
;
Jue YANG
1
;
Zhi-yin ZHANG
2
;
Wu-ling LIU
1
;
Hui SONG
1
;
Xiao-sen WU
3
;
Jing LI
3
;
Yan-mei LI
1
Author Information
1. The State Key Laboratory of Functions and Applications of Medicinal Plants， The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences，Guizhou Medical University，Guiyang 550014，China
2. Guiyang Hospital of Guizhou Aviation Industry Group，Guiyang 550027，China
3. Yongshun County Branch of Hunan Xiangxi Autonomous Prefecture Tobacco Company， Xiangxi Autonomous Prefecture 416000，China
- Publication Type:Research Article
- Keywords:
Jingulian capsule;
MDA-MB-231 cells;
apoptosis;
S phase arrest
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):78-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe effect of Jingulian capsule on the proliferation of human breast cancer MDA-MB-231 cells and investigate its action mechanism against triple negative breast cancer （TNBC）. Method:The ingredients of Jingulian capsule were identified by ultra-performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）. The inhibitory effect of Jingulian capsule at different doses （0.125，0.25，0.5，1，and 2 g·L-1） against the proliferation of MDA-MB-231 cells were detected by methyl thiazolyl tetrazolium （MTT） assay. After treatment for 24 h， the morphological changes in nuclear apoptosis of MDA-MB-231 cells were detected by Hoechst 33258 staining. The effect of different concentrations of Jingulian capsule on the apoptosis and cycle of MDA-MB-231 cells after different treatment time were determined by flow cytometry. The protein expression levels of Poly-ADP-ribose polymeras （PARP）， proto-oncogene c-Myc， cyclin B1， and phosphorylated extracellular signal-regulated kinase （p-ERK） in each group were assayed by Western blot. Result:A total of 113 compounds in Jingulian capsule were identified by UPLC-MS/MS. As revealed by MTT assay，compared with blank group，Jingulian capsule （0.125，0.25，0.5，1，2 g·L-1） significantly inhibited viability of MDA-MB-231 cells （P<0.01）， with the half maximal inhibitory concentration （ IC50） of（0.13±0.02）g·L-1. According to flow cytometry，compared with the blank group，Jingulian capsule at 1 g·L-1 significantly induced the apoptosis of MDA-MB-231 cells （P<0.05）and Jingulian capsule at 0.5， 1 g·L-1 obviously increased the number of MDA-MB-231 cells in S phase （P<0.05，P<0.01）. The results of Western blotting demonstrated that the protein expression levels of PARP，c-Myc，and cyclin B1 in 0.5， 1 g·L-1 Jingulian capsule groups were remarkably down-regulated as compared with those in the blank group（P<0.01）， and the protein expression level of p-ERK in 1 g·L-1 Jingulian capsule group was also down-regulated （P<0.01）. Conclusion:Jingulian capsule is able to inhibit the proliferation of MDA-MB-231 cells，induce S phase cell cycle arrest， and promote their apoptosis， which may be related to the inactivation of the MAPK signaling pathway.
