Immunoregulatory Effect of Chaihu Jia Longgu Mulitang Modified with Bupleuri Radix on Hippocampal NLRP3 Pathway in Depressed Rats
10.13422/j.cnki.syfjx.20202303
- VernacularTitle:柴胡加龙骨牡蛎汤对抑郁大鼠海马NLRP3通路的免疫调节作用
- Author:
Li-zhi SHANG
1
;
Meng-di MAO
1
;
Er-ping XU
1
;
Ming-yuan ZHANG
1
;
Chun-yu ZHOU
1
;
Guo-qiang WANG
1
;
Yao-yang LI
1
;
Bao-guang LIU
1
;
Ming BAI
1
;
Yu-cheng LI
1
;
Bao-ying WANG
1
;
Xiao-hui CHEN
1
Author Information
1. Academy of Chinese Medical Sciences，School of Basic Medical Science，The First Clinical School of Henan University of Chinese Medicine，Zhengzhou 450046，China
- Publication Type:Research Article
- Keywords:
depression;
Chaihu Jia Longgu Mulitang;
inflammation;
hippocampus;
NOD-like receptor protein 3（NLRP3）inflammasome
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):33-39
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect and mechanism of Chaihu Jia Longgu Mulitang （CJLM） on hippocampal NOD-like receptor protein 3 （NLRP3）inflammasome pathway in rats with depression. Method:Sixty male SD rats were randomly divided into a normal group，a model group， a MCC950 （1 mg·kg-1） group， and high- （13 g·kg-1）， medium- （6.5 g·kg-1）， and low-dose （3.25 g·kg-1） Chaihu Jia Longgu Mulitang groups， with 10 rats in each group．The depression model was induced by isolation combined with chronic unpredictable mild stimulation（CUMS） in rats except for those in the normal group. Rats were treated correspondingly for 21 days by intraperitoneal injection in the MCC950 group and gavage in other groups. The normal group and the model group received an equal volume of normal saline. The depression-like behaviors of rats were observed by sucrose preference test （SPT） and novelty-suppressed feeding test. Enzyme-linked immunosorbent assay （ELISA） was used to determine the levels of interleukin-1β （IL-1β） and IL-18 in the hippocampus of depressed rats. Western blot was used to detect the protein levels of NLRP3， apoptosis-associated speck-like protein containing a C-terminal caspase recruitment domain （ASC）， and Caspase-1. Result:Compared with the normal group， the model group showed decreased sucrose preference rate （P<0.01）， prolonged novelty-suppressed feeding time （P<0.01）， enhanced protein expression of NLRP3，ASC， and caspase-1 （P<0.05， P<0.01）， and elevated expression of IL-1β and IL-18 （P<0.01）. Conclusion:CJLM can alleviate depression-like behaviors in CUMS-induced model rats， and the underlying mechanism is related to the inhibition of the NLRP3 inflammasome pathway．
