Biological Evaluation for Quality Control of Water Extract of Qingjin Huatantang Based on Phagocytic and Secretory Functions of Macrophages
10.13422/j.cnki.syfjx.20211773
- VernacularTitle:基于巨噬细胞吞噬及分泌功能的清金化痰汤生物限值测定
- Author:
Qiong-ling ZHANG
1
;
Zheng-xiao SUN
1
;
Shun-li XIAO
1
;
Shi-lan DING
1
;
Jun XU
2
;
Tie-jun ZHANG
2
;
Yun YOU
1
Author Information
1. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
2. Tianjin Institute of Pharmaceutical Research，Tianjin 300301，China
- Publication Type:Research Article
- Keywords:
Qingjin Huatantang;
water extract;
lyophilized powder;
RAW264.7 macrophage;
phagocytic index;
interleukin;
bioactivity assessment
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(24):10-16
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for evaluating the biological activity of water extract lyophilized powder of Qingjin Huatantang based on the phagocytic and secretory functions of macrophages， and to control the quality of this formula from the biological activity level. Method:The phagocytic and inflammation models of RAW264.7 macrophages were established， the inhibition rates of water extract lyophilized powder of Qingjin Huatantang on interleukin-6 （IL-6） secretion and phagocytic index of neutral red of RAW264.7 macrophages were chosen as indicators to investigate the biological activity of Qingjin Huatantang， and the biological limit was searched. Result:The optimal inoculation density of RAW264.7 macrophages was 3×105 pcs/mL， and the concentration of lipopolysaccharide （LPS） was 1 mg·L-1 after treatment for 24 h. When the concentration was 500 mg·L-1， water extract lyophilized powder of Qingjin Huatantang had no toxicity and no obvious promotion effect on the proliferation of RAW264.7 macrophages， and at this concentration， the phagocytosis of RAW264.7 macrophages for neutral red was significantly promoted， the phagocytic index was >113%. In addition， the lyophilized powder had a significant and stable inhibitory effect on IL-6 secretion of RAW264.7 macrophages induced by LPS， the inhibitory rate was >45%. Conclusion:Combined with the anti-inflammatory and immunomodulatory effects of Qingjin Huatantang， this study establishes an in vitro biological limit method for evaluating the quality of water extract of Qingjin Huatantang based on the phagocytic and secretory functions of RAW264.7 macrophages， and 500 mg·L-1 was confirmed as the limit concentration. Under the limit concentration， Qingjin Huatantang water extract can significantly promote the phagocytic index of macrophages or significantly inhibit the secretion of IL-6 of RAW264.7 macrophages induced by LPS， which can be judged as qualified.
