Effect of Different Hot Air Drying Methods on Chemical Constituents of Trichosanthis Fructus
10.13422/j.cnki.syfjx.20211652
- VernacularTitle:不同热风干燥方式对瓜蒌化学成分的影响
- Author:
Huan-ran JIAO
1
;
Min-min ZHANG
1
;
Heng-qiang ZHAO
1
;
Wei LIU
1
;
Meng-jian LIU
2
;
Li CUI
1
Author Information
1. Shandong Analysis and Test Center，Qilu University of Technology （Shandong Academy of Sciences），Jinan 250014，China
2. Jinan Hebao Chinese Traditional Medicinal Co. Ltd.，Jinan 271100，China
- Publication Type:Research Article
- Keywords:
hot air drying;
Trichosanthis Fructus;
nucleosides;
volatile components;
gas chromatography-ion mobility spectrometry （GC-IMS）;
flavonoids
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):137-144
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To compare the effects of different drying methods on the chemical constituents of Trichosanthis Fructus. Method:Trichosanthis Fructus was dried by means of air drying， sun drying， hot air drying （40， 60， 80 ℃） and variable temperature drying （50-80， 80-50 ℃）. The contents of nucleosides and flavonoids in Trichosanthis Fructus peels and seeds treated by different methods were compared by high performance liquid chromatography （HPLC）， mobile phase was acetonitrile-0.2% acetic acid aqueous solution （3∶7） （A）-acetonitrile （B） for gradient elution （0-15 min， 97-95%B； 15-30 min， 95%-90%B； 30-35 min， 90%-87%B； 35-40 min， 87%-86.5%B； 40-48 min， 86.5%-97%B； 48-50 min， 97%B）， the detection wavelength was 260 nm， and the flow rate was 0.4 mL·min-1. Gas chromatography-ion mobility spectrometry （GC-IMS） was used to compare the changes of volatile components in the samples treated by different treatments. The volatile components were incubated on a SE-54 capillary column （0.32 mm×30 m， 0.25 μm） at 80 ℃ and 500 r·min-1 for 15 min， the injection temperature was 85 ℃， the injection volume was 400 μL， the analysis time was 35 min， carrier gas was high purity nitrogen， the flow rate of carrier gas was 2.0 mL·min-1， the flow rate of drift gas was 150 mL·min-1， and the temperature of IMS detector was 45 ℃. Result:The contents of uridine， adenosine and adenine were higher after hot air drying at >50 ℃. Low temperature drying was conducive to maintaining the stability of cytidine， cytosine， rutin， luteolin and 2ʹ-deoxyadenosine. GC-IMS technology could realize the analysis and identification of Trichosanthis Fructus samples after different treatments. There were more volatile components after hot air drying at 80 ℃ and variable temperature drying. Conclusion:Hot air drying at 40 ℃ and 60 ℃ can retain nucleosides and flavonoids， and the volatile components are similar to those in traditional drying methods， which has the advantages of high efficient， controllable and suitable for industrial production.
