Danshen Injection Inhibits SKOV3 Cell Proliferation in Vitro by Interfering with Their Interaction with Platelets
10.13422/j.cnki.syfjx.20212324
- VernacularTitle:丹参注射液通过干扰肿瘤细胞与血小板相互作用抑制SKOV3细胞体外增殖
- Author:
Ping YUAN
1
;
Si-qin JIANG
1
;
Yi-ran NIE
2
;
Sheng-ling LIU
3
;
Wei-ran FU
4
;
Hui-ru TIAN
1
;
Jian-jiang FU
1
Author Information
1. School of Pharmacy， Jiangxi University of Chinese Medicine， Nanchang 330004，China
2. International Education College， Jiangxi University of Chinese Medicine， Nanchang 330004，China
3. College of Life Sciences， Jiangxi University of Chinese Medicine， Nanchang 330004，China
4. School of Basic Medical Sciences， Capital Medical University， Beijing 100069，China
- Publication Type:Research Article
- Keywords:
Danshen injection;
platelet;
ovarian cancer;
interaction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):59-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the inhibitory effects of Danshen injection against ovarian cancer cell proliferation induced by the interaction between platelets and cancer cells. Method:The induction of platelets on SKOV3 growth in vitro and the inhibitory effect of Danshen injection at 12，24，and 48 g·L-1 were observed by 3-（4，5-Dimethylthiazol-2-yl）-2，5-diphenyltetrazolium bromide （MTT） and cell colony formation assays. The content of transforming growth factor-β1 （TGF-β1） in the platelet-tumor cell interaction system and platelet supernatant and the effect of Danshen injection on TGF-β1 secretion were detected by enzyme-linked immunosorbent assay （ELISA）. The influences of tumor cell culture supernatant on platelet aggregation and secretion and the inhibitory effect of Danshen injection were determined by microplate assay and ELISA. The effects of Danshen injection on platelet nuclear factor kappa B （NF-κB） signaling pathway were assayed by Western Blot. Result:Compared with the blank group， the platelet induction group exhibited significantly elevated absorbance at A570 （P<0.01）， while the absorbance at A570 in the platelet + Danshen injection group was significantly lower than that in the platelet induction group （P<0.01）. The comparison with the Danshen injection group revealed that the cell proliferation inhibitory rate in the platelet + Danshen injection group at the same dose was more significant （P<0.01）. The number of colonies in the platelet induction group was obviously increased in contrast to that in the blank group（P<0.05）， while the number of colonies in the platelet + Danshen injection group was significantly lower than that in the platelet induction group（P<0.05，P<0.01）. As demonstrated by comparison with the blank group， TGF-β1 content in the supernatant of the platelet induction group rose remarkably（P<0.01）， whereas that in the platelet + Danshen injection group declined（P<0.01）. Compared with the Danshen injection （24 g·L-1） group， the platelet + Danshen injection group displayed more obvious inhibition（P<0.01）. Compared with the blank group， Danshen injection significantly reduced the TGF-β1 content in platelet supernatant（P<0.05，P<0.01）. There was no significant change in the content of TGF-β1 in SKOV3 supernatant treated with Danshen injection. The platelet aggregation， thromboxane A2（TXB2）， and serotonin （5-HT） secretion in the SKOV3 cell supernatant induction group were significantly increased as compared with those in the blank group （P<0.01）， while such indexes in the cell supernatant induction + Danshen injection group were obviously decreased （P<0.01）. Compared with the Danshen injection （24 g·L-1） group， the cell supernatant induction + Danshen injection group displayed more obvious inhibition at the same dose（P<0.01）. Compared with the blank group， the platelet induction group exhibited obviously up-regulated phosphorylated TGF-β-activated kinase-1 （TAK-1） and NF-κB， but down-regulated phosphorylated inhibitory protein of NF-κB （IκB）（P<0.01）， which however were significantly reversed in the platelet + Danshen injection group（P<0.01）. Conclusion:Danshen injection affect the proliferation of SKOV3 cells by inhibiting their interaction with platelets， which may be related to the inhibited secretion of TGF-β1.
