Role of Counterevidence from Chinese Medicinal Prescription in Construction of Integrated Disease-syndrome Animal Model： Xiaoyaosan as Counterevidence for Depression of Liver Depression and Spleen Deficiency Syndrome
10.13422/j.cnki.syfjx.20212391
- VernacularTitle:方剂反证在构建病证结合动物模型中的作用
- Author:
Yu-bo LI
1
;
Mei YU
2
;
Jun-ling LI
3
;
Gai-mei HAO
1
;
Yuan LIANG
1
;
Zhi-guang ZHAI
1
;
Zhi-geng LI
1
;
Hai-hua JIA
1
;
Qing MIAO
1
;
Wei WANG
4
Author Information
1. Institute of Basic Theory of Traditional Chinese Medicine（TCM）， China Academy of Chinese Medical Sciences，Beijing 100700，China
2. Beijing Aerospace General Hospital，Beijing 100076，China
3. School of TCM，Capital Medical University，Beijing 100069，China
4. Guangzhou University of Chinese Medcine，Guangzhou 510006，China
- Publication Type:Research Article
- Keywords:
counterevidence from Chinese medicinal prescription;
integrated disease-syndrome;
animal model;
depression;
liver depression and spleen deficiency
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(23):44-50
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To seed for stable time window of the integrated disease-syndrome animal model based on the counterevidence from Chinese medicinal prescriptions， and to verify syndrome stability and reliability. Method:A model of depression was established by exposing rats to chronic unpredictable mild stress （CUMS）， followed by body weight measurement， sugar water test， behavioral test， and brain 5-hydroxytryptamine（5-HT） detection. The identification of liver depression and spleen deficiency syndrome was conducted after the equivalent transformation of human clinical symptoms into macroscopic representations of rats. Based on the dynamically collected macroscopic representation scale， Xiaoyaosan was used to reversely verify the stability and reliability of the integrated disease-syndrome animal model of depression due to liver depression and spleen deficiency. Result:The sugar water consumption and the number of crossings and the total movement distance in the open field test of 16-week-old rats in the CUMS （eight weeks of CUMS） group were significantly lower than those in the normal group （P<0.05）. According to the immunohistochemical results， the 5-HT content in hippocampal area CA2 of rats in the CUMS group was also significantly lowered as compared with that in the normal group（P<0.05），which indicated that depression was successfully modeled. The liver depression and spleen deficiency syndrome was present in 14-week-old rats （six weeks after CUMS）of the CUMS group， and the number of rats experiencing the liver depression and spleen deficiency syndrome reached the peak in the 16th week （eight weeks after CUMS），accounting for 70% of the total number. Thereafter， the number decreased gradually. The syndrome scores of the 14-， 16-， 18-， 20-， and 22-week-old rats in the Xiaoyaosan group were reduced by 66.6%， 70.7%， 54.8%， 50.4%， and 44.8%， which were graded as effective， marked effective， effective， effective， and effective， respectively. Conclusion:The age of 14-16 weeks（six to eight weeks after CUMS） is considered the stable and reliable time window for depression due to liver depression and spleen deficiency.
