Effect of TSG on GSK3β，PKA and PP2A of APP/PS1/Tau Triple-transgenic Mice Dementia Model
10.13422/j.cnki.syfjx.20202305
- VernacularTitle:二苯乙烯苷对APP/PS1/Tau三转基因小鼠痴呆模型GSK3β，PKA，PP2A的影响
- Author:
Yan-zhao SU
1
;
Wen-xue WU
2
;
Chao-yu LIU
2
;
Wan-ying MENG
1
;
Zhen-zhong LI
3
;
Jian HUANG
3
;
Xiao-ying ZHU
4
;
Yan-hua LIAO
4
;
Zhong-shi HUANG
2
Author Information
1. College of Pharmacy， Guangxi University of Chinese Medicine， Nanning 530200， China
2. College of Basic Medicine， Youjiang Medical University for Nationalities， Baise 533000， China
3. College of Pharmacy， Youjiang Medical University for Nationalities， Baise 533000， China
4. College of Clinical Medicine， Youjiang Medical University for Nationalities， Baise 533000， China
- Publication Type:Research Article
- Keywords:
tetrahydroxy stilbene glycoside;
Alzheimer's disease;
glycogen synthase kinase 3β（GSK3β）;
cAMP-dependent protein kinase（PKA）;
serine/threonine phosphatase 2A（PP2A）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(4):64-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of tetrahydroxy stilbene glycoside （TSG） on the expression of glycogen synthase kinase 3β （GSK3β）， cyclic adenosine monophosphate-dependent protein kinase （PKA） and Serine/threonine phosphatase 2A（PP2A） in the brain of amyloid precursor protein/presenilin-1/Tau （APP/PS1/Tau） triple-transgenic mice dementia model. Method:A total of forty-five 8-month-old APP/PS1/Tau transgenic mice were randomly divided into model group， positive control group （Huperzine-A， 0.15 mg·kg-1）， low， medium and high dose TSG groups （TSG， 0.033，0.1，0.3 g·kg-1）， with 9 mice in each group， and another nine C5B7L/6J mice of the same age were selected as normal control group. After 60 days of intragastric administration， the general structure of hippocampal neurons was observed by hematoxylin-eosin （HE） staining， immunohistochemical （IHC） was used to detect the expression of PKA protein in the brain of mice in each group， the mRNA expression levels of GSK3β， PKA and PP2A were detected by real time quantitative reverse transcription polymerase chain reaction （Real-time PCR）， and protein expression levels of GSK3β and PP2A were detected by Western blot. Result:Compared with the normal control group， the apoptosis level of neurons in the model group was significantly increased， the protein and mRNA expression levels of GSK3β and PKA were significantly increased （P<0.05， P<0.01）， and the protein and mRNA expression levels of PP2A were significantly decreased （P<0.05， P<0.01）. Compared with the model group， the apoptosis level of neurons in each treatment group was significantly down-regulated， the protein and mRNA expression levels of GSK3β and PKA were significantly down-regulated （P<0.05， P<0.01）， and the protein and mRNA expression levels of PP2A were significantly increased （P<0.05， P<0.01）. Conclusion:The mechanism of TSG in the treatment of Alzheimer's disease （AD） may be related to lowering the transcription and expression of GSK3β and PKA， increasing the transcription and expression of PP2A.
