Effect of Danggui Niantongtang on Key Factors of Extrinsic and Intrinsic Apoptotic Pathway in Synovial Tissue of Adjuvant Arthritis Rats
10.13422/j.cnki.syfjx.20202409
- VernacularTitle:当归拈痛汤对佐剂性关节炎大鼠滑膜组织内外凋亡途径关键因子的影响
- Author:
Jia-yu LI
1
;
Fan ZHAO
2
;
Qi-jin LU
1
;
Yi-si CAI
1
;
Hong-xin LIN
1
;
Gang LIU
3
;
Li-xia YUAN
1
Author Information
1. School of Traditional Chinese Medicine，Southern Medical University，Guangzhou 510515，China
2. Shenzhen Hospital of Guangzhou University of Chinese medicine （Futian），Shenzhen 518034， China
3. Nanfang Hospital of Southern Medical University， Guangzhou 510515， China
- Publication Type:Research Article
- Keywords:
Danggui Niantongtang;
rheumatoid arthritis;
adjuvant arthritis rats;
tumor necrosis factor receptor super family 6 （Fas）;
B-cell lymphoma-2 （Bcl-2）;
apoptosis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2021;27(4):1-7
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Danggui Niantongtang on the protein and mRNA expression of key regulatory factors of the extrinsic and intrinsic apoptotic pathway in synovial tissue of adjuvant arthritis （AA） rats， and to further explore the mechanism of Danggui Niantongtang in the prevention and treatment of rheumatoid arthritis. Method:The general condition of AA rats， including its body weight， were observed. The changes of toe volume were detected by toe volume meter. Histopathological changes of synovium of knee joint were observed by hematoxylin-eosin （HE） staining. The mRNA and protein expression levels of tumor necrosis factor receptor super family 6 （Fas）， Fas-associating protein with a novel death domain（FADD）， B-cell lymphoma-2 （Bcl-2）， Bcl-2-associated X protein （Bax） and cysteinyl aspartate specific proteinase Caspase-3 （Caspase-3） were detected by Real-time fluorescence quantitative polymerase chain reaction （Real-time PCR） and Western blot. Result:Compared with the normal group， the toe volume of the model group increased significantly （P<0.01）， with significantly proliferated synovial cells， significantly reduced mRNA and protein expression levels of Fas， FADD， Bax and Caspase-3 in synovial tissues（P<0.05， P<0.01）， and significantly increased Bcl-2 level （P<0.01）. Compared with the model group， the swelling degree of toes in Danggui Niantongtang group and Tripterygium group was significantly alleviated （P<0.01）， with significantly improved synovial hyperplasia， significantly increased mRNA and protein expression levels of Fas， FADD， Bax and Caspase-3 （P<0.05， P<0.01）， and significantly decreased expression levels of bcl-2 mRNA and protein （P<0.05， P<0.01）. Conclusion:Danggui Niantongtang can effectively reduce joint swelling and abnormal proliferation of synovial tissue in AA rats. Its mechanism may be related to regulating the expression of Fas， FADD， Bax， Bcl-2 and Caspase-3， and promoting the apoptosis of synovial cells.
