B Cells in Murine Cervical Lymph Nodes are Conventional B-2 Cells.
10.3346/jkms.2006.21.3.391
- Author:
Seung Geun YEO
1
;
Joong Saeng CHO
;
Dong Choon PARK
Author Information
1. Department of Obstetric and Gynecology, College of Medicine, The Catholic University of Korea, Suwon, Korea. dcpark@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
B-Lymphocytes;
Lymph Nodes;
Flow Cytometry;
Enzyme-linked Immunosorbent Assay;
Cell Proliferation
- MeSH:
Spleen/cytology;
Phenotype;
Mice, Inbred BALB C;
Mice;
Male;
Lymph Nodes/*cytology;
Immunoglobulin M/chemistry;
Flow Cytometry;
Enzyme-Linked Immunosorbent Assay;
Culture Media/pharmacology;
Cell Proliferation;
Cell Culture Techniques/methods;
B-Lymphocytes/*cytology;
Antigens/metabolism;
Animals
- From:Journal of Korean Medical Science
2006;21(3):391-396
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the characteristic features of cervical lymph node B cells to determine whether their behavior differs from that of B cells located elsewhere, because cervical lymph nodes may be exposed to continual antigenic stimulation from the naso- and/or oropharynx. B cells were isolated from cervical lymph nodes, spleen and peritoneal fluid of mice, cultured in medium, and exposed to various stimuli. The expression of various surface molecules characteristic of lymphoid B cells was assayed by flow cytometry, and immunoglobulin secreted into the culture supernatants was evaluated by enzyme-linked immunosorbent assay. B220+ cells were cultured in medium alone or with lipopolysaccharide, and their entrance into S phase in response to stimuli was measured by proliferative assays. Phenotypic characteristics of cervical lymph node B cells included CD5low, CD23high, CD43low, B7.1low, B7.2low, and Syndecan-1low. Unstimulated lymphoid B cells did not secrete immunoglobulin, but, upon stimulation, secretion of IgM was increased more than secretion of IgA and IgG. B cells actively entered S phase after 48 hr stimulation. These results show that B cells in cervical lymph nodes are conventional B2 cells, like splenic B cells.
