Bioinformatics Analysis of Up-regulated Differential Genes in Rats post Spinal Cord Injury
10.3969/j.issn.1006-9771.2019.06.001
- VernacularTitle:大鼠脊髓损伤后脊髓组织中上调差异基因的生物信息学分析
- Author:
Zhan-qiong ZHONG
1
;
Qing-cai XUE
1
;
Xiao-hong YI
1
;
Cong HUANG
1
;
Hai-yan YU
1
;
Wei-wei LIU
1
;
Ji-lan CHEN
1
;
Jun XIA
1
;
Xiao ZHANG
2
Author Information
1. School of Basic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, China
2. Preclinical Medicine of Chengdu Medical College, Chengdu, Sichuan 610500, China
- Publication Type:Research Article
- Keywords:
spinal cord injury;
up-regulated genes;
bioinformatics
- From:
Chinese Journal of Rehabilitation Theory and Practice
2019;25(6):621-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the differential expression and gene functions of up-regulated genes in rats with spinal cord injury. Methods:Female Sprague-Dawley rats' model of spinal cord injury was established with the modified Allen's method. Gene chip technology was used to detect the variation of differentially expressed genes in the spinal cord after spinal cord injury in rats. The differences in genes, functional localization and pathways were analyzed with gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway database. Results:The results of total RNA quality in spinal cord segment were qualified. Gene chip results showed that there were 1874 differentially up-regulated genes and 2348 differentially down-regulated genes. Bioinformatics was used to analyze differentially up-regulated genes in terms of biological processes, cellular components, and molecular functions. The differentially up-regulated genes were involving apoptosis, immune response, inflammation, etc., pathway analysis mainly showed the differentially up-regulated genes involved phosphoinositide 3-kinase protein kinase B signaling pathway and Toll-like receptor signaling pathways. Conclusion:Differentially up-regulated genes may be involved in secondary reactions following spinal cord injury, such as inflammation, immune response and hypoxia, and then further affect motor function and sensory function.
