Effects of Ultrashort Wave on Inflammatory of Acute Lung Injury and Related Signaling Pathway in Rats
10.3969/j.issn.1006-9771.2020.00.019
- VernacularTitle:超短波对急性肺损伤大鼠炎症反应的疗效及其机制
- Author:
Jun ZHOU
1
;
Dan-ni LIU
2
;
Li-qiong WANG
1
;
Qian WANG
1
;
Yi LI
1
;
Meng-jian QU
2
;
Jing LIU
2
;
Ya-hua ZENG
2
;
Cheng-qi HE
1
Author Information
1. Department of Rehabilitation, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
2. Department of Rehabilitation, the First Affiliated Hospital of University of South China, Hengyang, Hunan 421001, China
- Publication Type:Research Article
- Keywords:
acute lung injury;
ultrashort wave;
inflammatory;
nucleotide-binding oligomerization domain like receptor protein 3;
signaling pathway;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2020;26(10):1176-1181
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effects of ultrashort wave (USW) on lipopolysaccharide (LPS) induced acute lung injury (ALI) and nucleotide-binding oligomerization domain like receptor protein 3 (NLRP3) signaling pathway in rats. Methods:Twenty-four three-month-old male Sprague-Dawley rats were randomly divided into control group (n = 8), ALI group (n = 8) and USW group (n = 8). The ALI and USW groups were instilled with LPS to induce ALI, and the USW group was treated with ultrashort wave 0, four and eight hours after instillation, 15 minutes a time. Twenty-four hours after instillation, the lung tissue of the rats was measured the wet/dry mass ratio (W/D), and observed under HE staining. Serum levels of interleukin (IL)-1β and IL-18 were detected with ELISA. The mRNA and protein expression of NLRP3, caspase-1 and IL-1β in the lung tissue were detected with reverse transcription polymerase chain reaction and Western blotting, respectively. Results:W/D increased in ALI group compared with that of the control group (P < 0.05), and it decreased in USW group without significance compared with that of ALI group (P > 0.05). Lung injury score increased in ALI group compared with that of the control group (P < 0.05), and it decreased in USW group compared with that of ALI group (P < 0.05); as well as the serum IL-1β and IL-18, and mRNA and protein expression of NLRP3, caspase-1 and IL-1β. Conclusion:USW can alleviate the inflammatory of acute lung injury, which may associate with inhibiting of NLRP3 signaling pathway.
