Effect of Exercise on Expression of NeuN and SynapsinI in Cortex and Cognitive Function in Rats after Cerebral Ischemia-reperfusion
10.3969/j.issn.1006-9771.2021.11.006
- VernacularTitle:运动训练对脑缺血再灌注大鼠认知功能及前额皮层神经元核抗原和突触素1表达的效果
- Author:
Meng-yu LING
1
;
Yi-zhuo YANG
2
;
Shuai LIU
2
;
Chao-qun YE
1
Author Information
1. Air Force Clinical College, Anhui Medical University, Beijing 100142, China
2. Department of Rehabilitation, Air Force Medical Center, Beijing 100142, China
- Publication Type:Research Article
- Keywords:
ischemia-reperfusion;
cognitive impairment;
exercise;
SynapsinI;
NeuN;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2021;27(11):1272-1281
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of exercise on cognitive function and the expression of NeuN and SynapsinI in prefrontal cortex of rats after cerebral ischemia-reperfusion. Methods:Forty health male Sprague-Dawley rats were randomly divided into sham group (S group, n = 10), model group (M group, n = 10), sham & exercise group (SE group, n = 10) and model & exercise group (ME group, n = 10). The left middle cerebral artery was occluded for one hour and reperfused. SE and ME groups accepted treadmill training, for 14 days. The cognitive function of rats was evaluated with open field experiment and new object recognition experiment. The number and distribution of nerve cells in rat brain were observed with Nissl staining and immunofluorescence staining. The SynapsinI expression in serum was detected with ELISA. Results:Compared with S group, the activity, times of crossing, length and activity time in central area of open field experiment decreased in M group, as well as the cognitive index and length of new object recognition experiment (P < 0.05). Compared with M group, all the indexes increased in ME group (P < 0.05). The number of Nissl bodies decreased in M group (P < 0.05) and the arrangement was disordered, while the number of Nissl bodies increased in ME group (P < 0.05) compared with M group. The NeuN positive cells was more in S group than in M group (P < 0.05), and it was more in ME group than in M group (P < 0.05); as well as that of SynapsinI. The expression of SynapsinI decreased in M group (P < 0.05), and increased in ME group (P < 0.05). Most results of behavioral experiments positively correlated with the expression of Nissl, NeuN and SynapsinI (r > 0.221, P < 0.05). Conclusion:Cerebral ischemia-reperfusion can cause cognitive impairment in rats. Exercise can alleviate nerve injury and improve cognitive function, which may relate to promoting the expression of NeuN and SynapsinI in prefrontal cortex, to increase the number of neurons and synapses.
