Effect of Electroacupuncture on Blood-brain Barrier in SAMP8 Mice
10.3969/j.issn.1006-9771.2019.02.006
- VernacularTitle:电针对SAMP8小鼠血脑屏障的影响
- Author:
Zhi-xue YANG
1
;
Cheng-lin TANG
1
;
Xiao-hong LI
2
;
Zheng-wei ZHU
1
;
Si-qin HUANG
1
;
Ni DAI
1
;
Meng-jia WU
1
;
Li QIU
1
;
Hui-yu AN
1
Author Information
1. College of Traditional Chinese Medicine, Chongqing Medical University, Chongqing 400016, China
2. Department of Rehabilitation, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
- Publication Type:Research Article
- Keywords:
Alzheimer's disease;
electroacupuncture;
blood-brain barrier;
mice
- From:
Chinese Journal of Rehabilitation Theory and Practice
2019;25(2):156-161
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of electroacupuncture on blood-brain barrier to promote intake of drugs into brain of SAMP8 mice. Methods:The male SAMP8 mice (30-week-old) were randomly divided into model group (n = 7), drug group (n = 7), acupuncture group (n = 7) and combined group (n = 7). Other SAMR1 mice were as control group (n = 7). The acupuncture group and the combined group accepted electroacupuncture at Baihui (DU20) and Yintang (EX-HN3), the drug group and the combined group accepted Donepezil, for four weeks. They were observed hippocampus tight junction (TJ) under transmission electron microscope. The activities of acetylcholinesterase (AChE) in hippocampus were detected with immunohistochemistry. The mRNA expression of ZO-1, Claudin-5 and Occludin was detected with real-time quantitative polymerase chain reaction. Results:TJ and basement membrane in the control group, the acupuncture group and the combined group were better than those in the model group and the drug group. AChE was the most in the combined group, and then the drug group and the control group, the acupuncture group and the model group (P < 0.001). The expression of mRNA of ZO-1, Claudin-5 and Occludin was more in the acupuncture group and the combined group than in the drug group (P < 0.01). Conclusion:Electroacupuncture could ameliorate blood-brain barrier disruption and promote drug to enter the brain in SAMP8 mice, which may relate to the adjustion of ZO-1, Claudin-5 and Occludin.
