Mechanism of action of Xiaochaihu decoction in the treatment of hepatitis B based on network pharmacology
10.3969/j.issn.1001-5256.2021.10.010
- VernacularTitle:基于网络药理学研究小柴胡汤治疗乙型肝炎的作用机制
- Author:
Shaohang LAN
1
;
Qiuyuan TANG
1
,
2
;
Nana LI
1
;
Ran TAO
1
;
Nansheng LIAO
1
;
Yinjie MENG
1
;
Cao HE
2
;
Dewen MAO
1
,
2
Author Information
1. Graduate School, Guangxi University of Chinese Medicine, Nanning 530001, China
2. Department of Hepatology, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Nanning 530023, China
- Publication Type:Original articles_Viral hepatitis
- Keywords:
Hepatitis B;
XIAOCHAIHU DECOCTION;
Network Pharmacology;
Molecular Mechanisms of Pharmacological Action
- From:
Journal of Clinical Hepatology
2021;37(10):2308-2315
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of action of Xiaochaihu decoction in the treatment of hepatitis B based on network pharmacology. Methods The TCMSP database was used to obtain the main chemical components and action targets of the seven traditional Chinese medicines in Xiaochaihu decoction; the GeneCards and OMIM databases were used to obtain the targets associated with hepatitis B; the STRING online platform was used to construct a PPI network of potential targets, and R language was used to perform gene ontology (GO) functional enrichment analysis and KEGG pathway analysis; Cytoscape 3.7.2 was used to construct an "active component-core target" network and perform a topology analysis of this network; AutoDock vina and related software were used to perform molecular docking and visualized analysis of the active components with high value and the core targets in the network. Results A total of 193 main chemical components (including quercetin, kaempferol, wogonin, and naringenin) and 247 related targets were screened out, among which the key targets included RELA, MAPK1, TP53, ESR1, EGFR, and AKT1. A total of 2612 enrichment items were obtained by GO functional enrichment analysis, which were mainly involved in regulating the biological processes such as cell response to chemical stress, response to drugs, oxidative stress response, and lipopolysaccharide response. A total of 174 pathways were obtained by the KEGG pathway analysis, mainly involving hepatitis B, PI3K-AKT signaling pathway, and MAPK signaling pathway. Molecular docking results showed that the main active components had strong binding force to core targets, and the protein crystal complex had a stable conformation. Conclusion This study preliminarily shows that Xiaochaihu decoction exerts a therapeutic effect on hepatitis B through multiple components, targets, and pathways.
