Effect of silencing HDAC9 on the proliferation and differentiation of periodontal ligament stem cells
10.12016/j.issn.2096-1456.2022.02.003
- Author:
GE Wenbin
1
;
ZHANG Kun
1
;
LUO Shitong
1
;
ZHOU Zhi
2
;
LIU Yali
1
Author Information
1. Department of Orthodontics, Stomatological Hospital of Kunming Medical University
2. Department of Orthodontics, the Affiliated Hospital of Yunnan University
- Publication Type:Journal Article
- Keywords:
histone deacetylase 9;
periodontal ligament stem cells;
proliferation;
osteogenic differentiation;
proliferating cell nuclear antigen;
mineralized nodules;
runt-related transcription factor 2;
alkaline phosphatase
- From:
Journal of Prevention and Treatment for Stomatological Diseases
2022;30(2):89-96
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of silencing histone deacetylase 9 (HDAC9) expression on the proliferation and osteogenic differentiation of periodontal ligament stem cells (PDLSCs).
Methods: PDLSCs were isolated, cultured and identified in vitro. An siRNA construct specific for HDAC9 was transfected into PDLSCs (siHDAC9 group), and a nontargeting siRNA was used as a control (siNC group). The interference effect was determined by qRT-PCR. The cell cycle progression of PDLSCs was detected using flow cytometry. The proliferation activity of PDLSCs was detected via CCK-8 assay. Western blotting was used to detect the protein expression of proliferating cell nuclear antigen (PCNA). The mRNA expression of runt-related transcription factor 2 (RUNX2) and alkaline phosphatase (ALP) was investigated by qRT-PCR. The protein expression of RUNX2 was detected by western blotting. In addition, the formation of mineralized nodules was assessed by alizarin red staining.
Results:Compared with that in the siNC group, the mRNA expression of HDAC9 in the siHDAC9 group was lower (P < 0.01). Moreover, compared with those in the siNC group, the proliferation index (P<0.01), proliferation activity (P<0.05) and protein expression of PCNA (P<0.01) in the siHDAC9 group were all increased. Compared with the siNC group, the siHDAC9 group exhibited higher mRNA expression of RUNX2 and ALP (P < 0.05), and the protein expression of RUNX2 showed the same results (P < 0.01). The results of alizarin red staining showed that compared to the siNC group, the siHDAC9 group formed more mineralized nodules.
Conclusion:Silencing HDAC9 expression can promote the proliferation and osteogenic differentiation of PDLSCs.
- Full text:沉默HDAC9对牙周膜干细胞增殖与成骨分化的影响.pdf
