Anti-tumor activities of endophytes from Cynanchum bungei Decne.
10.12206/j.issn.1006-0111.202108089
- VernacularTitle:泰山白首乌内生真菌多样性及其抗肿瘤活性研究
- Author:
Kunmiao FENG
1
;
Sijia WU
1
;
Wenhua CHEN
2
;
Wei DAI
3
;
Lingchuan XU
3
;
Ting HAN
1
Author Information
1. School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou 310053, China.
3. School of Pharmacy, Shandong Chinese Medical University, Jinan 250355, China.
- Keywords:
C. bungei;
Alternaria;
anti-tumor activity
- From:
Journal of Pharmaceutical Practice
2021;39(6):542-548
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the taxonomic structure and diversity of endophytic fungi from Cynanchum bungei Decne., explore the potential microbial resources and functions and provide the theoretical basis for new antitumor endophytic fungi. Methods The diversities of endophytic fungi community in different tissues, species and habitats were analyzed with traditional endophytic bacteria separation method and 18sRNA high-throughput sequencing technology. MTT assay was used to detect the cytotoxic activity of endophytic fungi from Radix Polygoni multiflori. Results 90 strains of endophytic fungi were isolated and identified from roots, stems, and leaves of C. bungei. Among them, Fusarium and Alternaria were the dominant genera. There were 8, 9 and 13 genera from roots, stems and leaves of C. bungei respectively. Among which Alternaria and Colletotrichum were the common genera in different tissues. Further studies showed that 13 endophytic fungi of C.bungei had good anti-tumor activity in vitro, accounting for 14.4% of the total genera. Among them, A. tenuissima LTJ2 and A. alternata LTJ6 had significant anti-tumor activity. Conclusion The endophytic fungi in Cynanchum bungei Decne. have rich diversity. Some strains have significant anti-tumor activity，which can be potential resources for the development of new antitumor agents.
