Effect of different injection approaches of tumor RNA nanoliposome vaccine on the growth of colon cancer
10.12206/j.issn.1006-0111.202108094
- VernacularTitle:不同途径注射肿瘤RNA纳米脂质体疫苗对结肠癌生长的影响
- Author:
Guangzhao LU
1
;
He ZHANG
1
;
Li FAN
1
;
Zhiguo SUN
1
;
Ying LU
1
Author Information
1. Department of Pharmaceutical Sciences, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
- Keywords:
CT26 colon cancer cells;
nanoliposomes;
vaccine;
tumor RNA
- From:
Journal of Pharmaceutical Practice
2021;39(6):520-524
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the differences in the anti-tumor growth effects of organisms with different injections of CT26 tumor cell RNA loaded into nanoliposomes. Methods The extracted tumor RNA was loaded into nanoliposomes to prepare tumor RNA nanoliposome vaccines, and the related properties of nanoliposome vaccines were investigated. The particle size of nanoliposome vaccines was （120.0±12.1）nm and zeta potential was （3.39±0.56）mV. Tumor RNA nanoliposome vaccines were injected into different parts of the mice to test and analyze the influence of different injections on the growth of colon cancer transplanted tumors in mice. Results Tumor RNA nanoliposome vaccines were used to inject tumor-transplanted mice in different ways. Compared with underarm injection, intraperitoneal injection enhanced the organism's anti-tumor immune response and inhibited the growth of transplanted tumors more effectively. The H&E staining of important organs in mice was compared and no obvious organic lesions were found in the organs. Conclusion Intraperitoneal injections of nanoliposome loaded with tumor RNA can enhance the body's anti-tumor immune response more effectively than underarm injections.
