Hops extract alleviates Aβ-injury to osteoblasts through antioxidant pathway
10.12206/j.issn.1006-0111.202103018
- VernacularTitle:啤酒花经抗氧化途径减轻Aβ损伤成骨细胞作用研究
- Author:
Tianshuang XIA
1
;
Xiaoyan LIU
1
;
Yiping JIANG
1
;
Xiaojin LI
2
;
Guoping WANG
2
;
Hailiang XIN
1
Author Information
1. Department of Pharmacognosy, School of Pharmacy, Naval Medical University, Shanghai 200433, China.
2. Xinjiang Institute of Traditional Chinese Medicine, Urumqi 830002, China.
- Keywords:
Hops (Humulus lupulus L.);
Aβ;
osteoblast;
antioxidation;
osteoporosis
- From:
Journal of Pharmaceutical Practice
2021;39(6):509-514
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of Humulus lupulus L. extract (HLE) and its mechanism on improving bone formation of Aβ-injured osteoblasts. Methods Osteoblasts isolated from 24 h-old Wistar rats were injured by Aβ1-42 oligomer and intervened with HLE. The proliferation, differentiation and bone mineralization of osteoblasts were determined by MTT assay, alkaline phosphatase (ALP) activity assay and alizarin red staining, respectively. The apoptosis of osteoblasts was detected by flow cytometer. The expression levels of bone formation related proteins, and proteins of Nrf2 and FoxO1 pathways were measured by Western blotting analysis. The intranuclear expression of FoxO1 protein was detected by immunofluorescence. Results HLE significantly improved the cell proliferation, ALP activity and bone mineralization, and inhibited the apoptosis of Aβ-injured osteoblasts. HLE also significantly promoted the expressions of collagen type Ι (COL-I) and osteopontin (OPN) in Aβ-injured osteoblasts. HLE notably activated the Nrf2 and FoxO1 signaling pathways in Aβ-injured osteoblasts by promoting the expressions of related proteins and maintained bone metabolism through relieving oxidative stress. Conclusion This study confirms that HLE can alleviate Aβ-injury to osteoblasts, and preliminarily clarifies the mechanism being related to antioxidation, which provides a new reference for the mechanism research and drugs development for anti-osteoporosis.
