Analysis of protein differences in aortic aneurysm/dissection based on tandem mass tag proteomics
10.7507/1007-4848.202101075
- VernacularTitle:基于串联质谱标签定量蛋白质组学筛选胸主动脉瘤/夹层蛋白质标志物
- Author:
Yu ZHANG
1
;
Hongwei ZHANG
1
;
Peng YANG
1
;
Chen LU
1
;
Yu LIU
1
;
Haiyue WANG
1
;
Jia HU
1
Author Information
1. partment of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, 610041, P.R.China
- Publication Type:Journal Article
- Keywords:
Tandem mass tag proteomics;
aortic aneurysm;
aortic dissection;
medial degeneration
- From:
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
2021;28(10):1222-1228
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the differences in proteins between aneurysm/dissection patients and healthy subjects, and subsequently figure out differential proteins related to medial degeneration of aortic aneurysm/dissection. Methods Aortic wall samples were collected from 6 male aortic aneurysm patients (an aortic aneurysm group, mean age 56.50±8.19 years), 6 male aortic dissection patients (an aortic dissection group, mean age 54.17±6.68 years) and 6 male healthy subjects (a normal group, mean age 40.50±9.31 years) between December 2019 and May 2020 in West China Hospital of Sichuan University. Quantitative proteomics was performed using tandem mass tag (TMT) techniques, followed by gene ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Results A total of 63 differential proteins were obtained both in the aortic aneurysm group and the aortic dissection group compared with the normal group, with 30 up-regulating and 33 down-regulating. The differential proteins were involved in multiple biological processes and clusted on peroxisome proliferators-activated receptor (PPAR) signaling pathway, extracellular matrix-receptor interaction signaling pathway and complement and coagulation cascades signaling pathway. Conclusion The identified proteins may help to demonstrate new molecular mechanisms related to medial degeneration of aortic aneurysm/dissection.
